Isolation of microsatellite loci from the onion thrips, Thrips tabaci
Isolation of microsatellite loci from the onion thrips, Thrips tabaci
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DOI：
10.1673/031.013.3001
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发表时间：
2013-04-18
影响因子：
2.2
通讯作者：
Hasegawa, Eisuke
中科院分区：
文献类型：
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作者：
Kobayashi, Kazuya;Hasegawa, Eisuke
烟蓟马（Thrips tabaci Lindeman）是一种世界性害虫，至少可分为3个遗传谱系，它们具有不同的生物学特性，如繁殖方式和对杀虫剂的耐受性。由于谱系仅通过线粒体DNA来区分，因此在基因组水平上谱系之间存在基因流动的可能性。从该类群中新分离到9个多态性微卫星位点，在51个个体中观察到中度到高度多态性，等位基因数从6到12不等。平均观察到的和预期的杂合性范围分别为0.1373至0.3725和0.5381至0.7748。与Hardy-Weinberg平衡下的预期相反，9个位点中有6个表现出纯合性降低。然而，我们证实，在所有的基因座的等位基因遗传的孟德尔特征。这些新的位点将有助于探讨该物种的谱系歧视和群体遗传学。
Thrips tabaci Lindeman (Thysanoptera: Thripidae), a cosmopolitan pest insect, is subdivided into at least three genetic lineages that have different biological characters, such as reproductive mode and resistibility to insecticides. Since the lineages are discriminated only by mitochondrial DNA, there is a possibility of gene flow among lineages at the genomic level. Nine polymorphic microsatellite loci were newly isolated from the taxon. Moderate to high levels of polymorphism were observed, with numbers of alleles ranging from 6 to 12 in 51 individuals. The mean observed and expected heterozygosities ranged from 0.1373 to 0.3725 and 0.5381 to 0.7748, respectively. Contrary to the expectation under Hardy-Weinberg's equilibrium, six of the nine loci exhibited a reduction to homozygosities. However, we confirmed that alleles in all the loci were inherited as Mendeilan characteristics. These new loci will be useful to explore discrimination of lineages and population genetics in this species.