Differentiation of adult-type Leydig cells occurs in gonadotrophin-deficient mice.
Differentiation of adult-type Leydig cells occurs in gonadotrophin-deficient mice.
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DOI：
10.1186/1477-7827-1-4
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发表时间：
2003-02-05
期刊：
影响因子：
--
通讯作者：
O'Shaughnessy, P J
中科院分区：
文献类型：
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作者：
Baker, P J;Johnston, H;O'Shaughnessy, P J
在哺乳动物睾丸发育过程中，会产生不同代的胎儿和成人间质细胞。黄体生成素 (LH) 是正常成体间质细胞功能和正常成体间质细胞数量建立所必需的，但其在成体间质细胞分化过程中的作用仍不确定。在这项研究中，我们检查了循环促性腺激素缺乏的促性腺激素释放激素 (GnRH) 缺失小鼠的成年 Leydig 细胞分化。通过测量编码小鼠中成体间质细胞分化的四种特定标记物的 mRNA 种类的表达来评估成体间质细胞分化。这些标记物（VI 型 3β-羟基类固醇脱氢酶 (3betaHSD VI)、III 型 17β-羟基类固醇脱氢酶 (17betaHSD III)、前列腺素 D (PGD)-合成酶和雌激素磺基转移酶 (EST)）中的每一种仅在正常成年动物的成年 Leydig 细胞谱系中表达。实时 PCR 研究表明，所有四种标记物均在成年 GnRH 缺失小鼠中表达。通过原位杂交对 3betaHSD VI 和 PGD 合成酶表达的定位证实了这些基因在 GnRH 缺失小鼠的间质组织中表达。用人绒毛膜促性腺激素治疗动物在12小时内增加了3betaHSD VI和17betaHSD III的表达，进一步表明分化但未刺激的细胞已经存在于GnRH缺失小鼠中。因此，虽然之前的研究表明 LH 是成体 Leydig 细胞增殖和活性所必需的，但本研究的结果表明，成体 Leydig 细胞分化将在缺乏 LH 的动物中发生。
During mammalian testis development distinct generations of fetal and adult Leydig cells arise. Luteinising hormone (LH) is required for normal adult Leydig cell function and for the establishment of normal adult Leydig cell number but its role in the process of adult Leydig cell differentiation has remained uncertain. In this study we have examined adult Leydig cell differentiation in gonadotrophin-releasing hormone (GnRH)-null mice which are deficient in circulating gonadotrophins. Adult Leydig cell differentiation was assessed by measuring expression of mRNA species encoding four specific markers of adult Leydig cell differentiation in the mouse. Each of these markers (3beta-hydroxysteroid dehydrogenase type VI (3betaHSD VI), 17beta-hydroxysteroid dehydrogenase type III (17betaHSD III), prostaglandin D (PGD)-synthetase and oestrogen sulphotransferase (EST)) is expressed only in the adult Leydig cell lineage in the normal adult animal. Real-time PCR studies showed that all four markers are expressed in adult GnRH-null mice. Localisation of 3betaHSD VI and PGD-synthetase expression by in situ hybridisation confirmed that these genes are expressed in the interstitial tissue of the GnRH-null mouse. Treatment of animals with human chorionic gonadotrophin increased expression of 3betaHSD VI and 17betaHSD III within 12 hours further indicating that differentiated, but unstimulated cells already exist in the GnRH-null mouse. Thus, while previous studies have shown that LH is required for adult Leydig cell proliferation and activity, results from the present study show that adult Leydig cell differentiation will take place in animals deficient in LH.