Immunomodulatory effects of crude phenylethanoid glycosides from Ligustrum purpurascens
Immunomodulatory effects of crude phenylethanoid glycosides from Ligustrum purpurascens
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紫女贞粗苯乙醇苷的免疫调节作用
DOI：
10.1016/j.jep.2012.09.047
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发表时间：
2012-12-18
影响因子：
5.4
通讯作者：
He, Zhen-dan
中科院分区：
文献类型：
--
作者：
Song, Xun;Li, Chen-yang;He, Zhen-dan
民族药理学相关性：女贞子（Ligustrum purpurascens）又名苦丁茶，是我国南方传统的功能茶，具有利尿、降压、减肥、抗炎等功效。材料与方法：采用小鼠血清溶血素抗体测定、小鼠吞噬功能测定、小鼠脾细胞抗体产生测定、小鼠NK细胞活性测定等方法，研究CPGs对小鼠免疫功能的增强作用。结果：在免疫实验中，不同剂量的CPGs处理组小鼠的血凝效价均较对照组显著升高（P < 0.01）; 440 mg/kg和132 g/kg剂量组的脾细胞抗体生成、鸡红细胞吞噬M Phi细胞和NK细胞活性显著增加（p < 0.05）。进一步的化学结构鉴定得到10个化合物，其中5个苷类化合物经HPLC定量，另外5个化合物的结构根据紫外光谱和质谱推测。结论：女贞子中的苯乙醇苷类化合物具有免疫调节作用。皇冠版权所有（C）2012由爱思唯尔爱尔兰有限公司出版。保留所有权利。
Ethnopharmacological relevance: Ligustrum purpurascens, named as "Ku ding cha", has been used as a kind of functional tea in southern China for about two thousand years, Which has the effects on diuresis, anti-hypertension, weight-loss and anti-inflammation.The aim of the study: This study was aimed to investigate the immune enhancement effects of the crude phenylethanoid glycosides (CPGs) from Ligustrum. Purpurascens on mice and analyze the chemical profiles of phenylethanoid glycosides in the CPGs.Materials and methods: The immune functions enhancing potential of CPGs was determined using serum hemolysin antibody, phagocytosis, splenocyte antibody production, and NK cells activity assays. The contents of five major constituents in the crude glycosides of Ligustrum purpurascens were determined by using liquid chromatography, other five glycosides were deduced according to their UV and MS spectra compared with the literature as well.Results: In the immunizing experiment, mice treated with different doses of CPGs showed an increase (p < 0.01) in the haemagglutination titre compared with the control group. The increases (p < 0.05) were found to be significant at doses of 440 mg/kg and 132 g/kg in the experiments of antibody production of spleen cells, M Phi phagocytosis of chicken RBCs and NK cell activity. Further chemical characterization yielded 10 constituents from CPGs, five glycosides were quantified by HPLC and the structures of other five compounds were speculated according to their UV and MS spectra.Conclusion: The results suggested that phenylethanoid glycosides from Ligustrum purpurascens have immunomodulatory effects on mice. Crown Copyright (C) 2012 Published by Elsevier Ireland Ltd. All rights reserved.