PERIOVULATORY MULTIFOCAL MESOTHELIAL PROLIFERATION - A POSSIBLE ASSOCIATION WITH MALIGNANT TRANSFORMATION
PERIOVULATORY MULTIFOCAL MESOTHELIAL PROLIFERATION - A POSSIBLE ASSOCIATION WITH MALIGNANT TRANSFORMATION
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DOI：
10.1046/j.1525-1438.1995.05040306.x
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发表时间：
1995-07-01
影响因子：
4.8
通讯作者：
BENSASSON, S
中科院分区：
文献类型：
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作者：
BELLER, U;HAIMOVITCH, R;BENSASSON, S
本研究的目的是在确定的网站和组织更新的动力学特征在修复卵巢表面上皮（OSE）排卵周围。46 h后腹腔注射孕马血清促性腺激素（PMSG，5 IU）+/-人绒毛膜促性腺激素（HCG，5 IU），或皮下注射（sc）溶于0.5 ml玉米油的0.1 mg或1.0 mg二乙基司贝甾醇（DES），以及注射生理盐水的对照组，诱导青春期前C57 BL雌性小鼠发情。动物在不同的时间间隔后被杀死。在甲醛固定的生殖器官石蜡切片上进行放射自显影，随后进行苏木精和伊红染色。检查OSE以及周围和更远端间皮细胞中的细胞核[H-3]胸苷掺入（在杀死前1小时注射0.5 μ Ci）。令人惊讶的是，一个显着的增殖反应中观察到的卵巢外间皮周围的子宫角，管和附近的腹膜，而OSE的增殖率是不到一半的it. In对比常见的概念，它似乎是扩大和修复的OSE，与排卵，主要是由其卵巢外间皮连续。这一观察结果可能为卵巢癌发病机制中的多灶性理论提供额外的支持。因此，任何与恶性转化相关的细胞或分子事件的检索，除了OSE外，还应包括近端和远端间皮瘤。
This study was aimed at the identification of the site and characterization of the kinetics of tissue renewal during the repair of the ovarian surface epithelium (OSE) surrounding ovulation. Prepubescent C57BL female mice were induced hormonally either by intraperitoneal (ip) injection of pregnant mare serum gonadotropin (PMSG, 5 IU) +/- human chorionic gonadotropin (HCG, 5 IU) 46 h later, or subcutaneous (sc) diethylsilbesterol (DES) 0.1 mg or 1.0 mg in 0.5 ml corn oil, in addition to saline-injected controls. The animals were killed after various time intervals. Autoradiography was carried out on formaldehyde-fixed paraffin sections of genital organs with subsequent hematoxylin and eosin staining. The preparations were examined for nuclear [H-3]thymidine incorporation (0.5 mu Ci injected 1 h before killing) in the OSE, as well as the surrounding and more distal mesothelium. Surprisingly, a significant proliferative response was observed in the extra-ovarian mesothelium surrounding the uterine horns, tubes and nearby peritoneum, while the OSE proliferation rate was less than half of it. In contrast to the common notion, it seems that the expansion and repair of the OSE, associated with ovulation, is contributed mainly by its extra-ovarian mesothelial continuum. This observation may provide additional support to the multifocal theory suggested in the pathogenesis of ovarian cancer. Therefore, any search for cellular or molecular events related to malignant transformation should include the proximal and distal mesothelium in addition to the OSE.