Defoliation, not gibberellin, induces tree peony autumn reflowering regulated by carbon allocation and metabolism in buds and leaves.
Defoliation, not gibberellin, induces tree peony autumn reflowering regulated by carbon allocation and metabolism in buds and leaves.
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DOI：
10.1016/j.plaphy.2020.04.005
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发表时间：
2020-04
期刊：
影响因子：
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通讯作者：
Xiaoping Wang;Shunli Wang;Yu-qian Xue;Xiuxia Ren;Jingqi Xue;Xiuxin Zhang
中科院分区：
文献类型：
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作者：
Xiaoping Wang;Shunli Wang;Yu-qian Xue;Xiuxia Ren;Jingqi Xue;Xiuxin Zhang
短而集中的自然荧光阻碍牡丹的周年生产，秋季采用落叶和施用赤霉素（GA）的方法诱导牡丹开花。本文通过监测芽和叶的形态和可溶性糖的变化，以及研究碳分配和代谢相关基因的表达，确定了落叶和GA处理的个体作用。去叶和GA处理诱导早期芽发育，但诱导更快使用GA处理。只有去叶处理而非GA处理诱导了最终的再开花，尽管它们的组合促进了再开花，而且去叶处理比GA处理更快地降低芽中的蔗糖含量。蔗糖的减少可能在牡丹的再花过程中起着关键作用，而落叶后幼叶较高的碳代谢活性可能进一步促进了牡丹的再花过程。去叶处理增强了蔗糖转运蛋白PsSUT 4和PsSWEET 12在芽中的表达，且其在幼叶中的表达量大于GA处理。这表明PsSUT 4和PsSWEET 12可能在脱叶后帮助将碳运输到芽中。此外，转化酶PsCIN2和PsCWIN1在脱叶后幼叶中的表达量增加，表明它们可能通过促进幼叶蔗糖水解而参与脱叶后的再开花。此外，GA处理对叶片中PsVIN 1和PsVIN 2以及芽中PsVIN 2的表达量的诱导作用强于去叶处理，表明PsVIN可能主要受GA处理的影响。研究结果可为牡丹促成栽培技术及相关产业化生产提供参考。
Short and concentrated natural fluorescence hinders tree peony (Paeonia suffruticosa) annual production, and defoliation and gibberellin (GA) application is used to induce its reflowering in autumn. Here, the individual roles of defoliation and GA treatment were determined by monitoring morphological and soluble sugar changes in buds and leaves, and by investigating carbon allocation- and metabolism-related gene expression. Both defoliation and GA treatment induced early bud development, but induction was faster using the GA treatment. Only defoliation, not GA treatment, induced the final reflowering, although their combination accelerated it. Furthermore, defoliation decreased the sucrose content in buds much faster than the GA treatment. This sucrose reduction may play a key role in tree peony reflowering, and the higher carbon metabolism activity in young leaves after defoliation may further help the reflowering process. Defoliation enhanced the expression of sucrose transportersPsSUT4andPsSWEET12in buds, and their expression in young leaves was greater than after GA treatment. This indicated thatPsSUT4andPsSWEET12may help transport carbon into buds after defoliation. In addition, the invertases,PsCIN2andPsCWIN1in young leaves were more highly expressed after defoliation, indicating that they may contribute to reflowering after defoliation by accelerating sucrose hydrolysis in young leaves. In addition, the expression levels ofPsVIN1andPsVIN2in leaves, andPsVIN2in buds were more highly induced by GA treatment than by defoliation, indicating thatPsVINsmay mainly respond to GA treatment. These results may help improve the tree peony forcing culture technology and related industrial production.