A TonB-dependent transducer is responsible for regulation of pathogenicity-related genes in Xanthomonas axonopodis pv. citri
A TonB-dependent transducer is responsible for regulation of pathogenicity-related genes in Xanthomonas axonopodis pv. citri
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DOI：
10.1007/s10327-010-0227-4
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发表时间：
2010-04-01
影响因子：
1.2
通讯作者：
Tsuyumu, Shinji
中科院分区：
文献类型：
--
作者：
Aini, Luqman Qurata;Hirata, Hisae;Tsuyumu, Shinji
TonB依赖性转导子是几种植物病原细菌致病性所必需的。在这项研究中，我们研究了一个假定的TonB依赖的换能器，XAC4131，在Xanthomonaxonopodis pv。柑橘溃疡病的病原体。XAC4131基因的突变导致烟草叶片中过敏反应的诱发延迟。但在柑橘叶片中的致病性与野生型相似。在hrp诱导培养基中，XAC 4131控制hrp调节基因hrpG的表达。此外，XAC 4131参与了相邻基因rpoE（XAC 4129）和XAC 4130的表达，所述相邻基因分别编码RpoE（XAC 4129）和FecR样蛋白。结果表明，XAC 4131可能通过rpoE（XAC 4129）和XAC 4130间接控制hrpG的表达。此外，我们还证明了rpoE（XAC4129）、XAC4130和XAC4131的转录似乎受Fur蛋白的调控。
The TonB-dependent transducer is required for plant pathogenicity in several plant pathogenic bacteria. In this study, we investigated the role of a putative TonB-dependent transducer, XAC4131, in Xanthomonas axonopodis pv. citri, the causal agent of citrus canker disease. A mutation in the XAC4131 gene caused a delay in the elicitation of the hypersensitive reaction in tobacco leaves. However, the pathogenicity in citrus leaves was similar to that of wild type. In hrp-inducing medium, XAC4131 controlled the expression of the hrp regulatory gene hrpG. Also, XAC4131 was involved in expression of the adjacent genes rpoE (XAC4129) and XAC4130, which encode RpoE(XAC4129) and FecR-like protein, respectively. The results suggest that XAC4131 controls the expression of hrpG indirectly, probably via rpoE (XAC4129) and XAC4130. Furthermore, we also demonstrated that transcription of rpoE (XAC4129) , XAC4130 and XAC4131 seems to be regulated by the Fur protein.