Antisense to the glucocorticoid receptor in hippocampal dentate gyrus reduces immobility in forced swim test
Antisense to the glucocorticoid receptor in hippocampal dentate gyrus reduces immobility in forced swim test
复制标题
DOI：
10.1016/0014-2999(96)00064-7
复制
发表时间：
1996-04-22
影响因子：
5
通讯作者：
Bohus, B
中科院分区：
文献类型：
--
作者：
Korte, SM;DeKloet, ER;Bohus, B
将靶向糖皮质激素受体 mRNA 的 18 聚体反义硫代磷酸寡脱氧核苷酸双侧输注至海马齿状回后，强迫游泳试验中大鼠的不动时间减少。在初始测试期间，载体、有义序列和乱序序列处理的动物比反义处理的动物花费更多的时间不动。脑切片中糖皮质激素受体的免疫标记表明，与对侧有义处理的齿状回或对侧乱序处理的齿状回相比，反义处理的齿状回中糖皮质激素受体蛋白的表达减少。在初始测试期间，当大鼠提前 6 小时（但不是 1 小时）接受糖皮质激素受体拮抗剂 RU38486（17 beta-羟基-11 beta-（4-二甲氨基-苯基）17 α-（1-丙炔基）estra-4,9-diene-3-one））治疗时，静止时间也减少了。这些结果证明了糖皮质激素受体在初始测试期间参与强迫游泳休息的不动表达。
Immobility time of rats in the forced swim test was reduced after bilateral infusion of an 18-mer antisense phosphorothioate oligodeoxynucleotide targeted to the glucocorticoid receptor mRNA into the dentate gyrus of the hippocampus. Vehicle-, sense- and scrambled sequence-treated animals spent significantly more time immobile than antisense-treated animals during the initial test. Immunolabeling of the glucocorticoid receptor in brain sections demonstrated a reduced expression of glucocorticoid receptor proteins in antisense-treated dentate gyrus compared to the contralateral sense-treated dentate gyrus or contralateral scrambled sequence-treated dentate gyrus. During the initial test the time spent on immobility was also reduced when rats were treated with the glucocorticoid receptor antagonist RU38486 (17 beta-hydroxy-11 beta-(4-dimethylamino-phenyl)17 alpha-(1-propynyl)estra-4,9-diene-3-one)) 6 h (but not 1 h) earlier. These results demonstrate the participation of glucocorticoid receptors in the expression of immobility in a forced swim rest during the initial test.