Phosphatidylserine on HIV envelope is a cofactor for infection of monocytic cells
Phosphatidylserine on HIV envelope is a cofactor for infection of monocytic cells
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DOI：
10.4049/jimmunol.170.9.4840
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发表时间：
2003-05-01
影响因子：
4.4
通讯作者：
Henderson, AJ
中科院分区：
文献类型：
--
作者：
Callahan, MK;Popernack, PM;Henderson, AJ
HIV-1 是一种有包膜逆转录病毒，当病毒粒子离开细胞时，它会获得外膜。由于细胞凋亡与 AEDS 进展相关，HIV-1 感染的 T 细胞或巨噬细胞可能会表达升高水平的表面磷脂酰丝氨酸 (PS)，这是程序性细胞死亡的标志。这些细胞产生的病毒粒子也被预测在其包膜表面具有 PS。在这项研究中，提供的数据支持这一假设并表明 PS 是巨噬细胞感染所必需的。 PS 特异性蛋白膜联蛋白 V 用于富集病毒颗粒并抑制原代巨噬细胞中的 HIV-1 复制，但不能抑制 T 细胞。由 PS 组成的囊泡也显着抑制了 HIV-1 的复制，但磷脂酰胆碱则没有。 PS是HIV-1感染特别需要的，因为用水疱性口炎病毒G和双嗜性鼠白血病病毒包膜假型的病毒不受PS囊泡或膜联蛋白V的抑制。这些数据表明PS是巨噬细胞的HIV-1感染的重要辅助因子。
HIV-1 is an enveloped retrovirus that acquires its outer membrane as the virion exits the cell. Because of the association of apoptosis with the progression of AEDS, HIV-1-infected T cells or macrophages might be expected to express elevated levels of surface phosphatidylserine (PS), a hallmark of programmed cell death. Virions produced by these cells would also be predicted to have PS on the surface of their envelopes. In this study, data are presented that support this hypothesis and suggest that PS is required for macrophage infection. The PS-specific protein annexin V was used to enrich for virus particles and to inhibit HIV-1 replication in primary macrophages, but not T cells. HIV-1 replication was also significantly inhibited with vesicles consisting of PS, but not phosphatidylcholine. PS is specifically required for HIV-1 infection because viruses pseudotyped with vesicular stomatitis virus G and amphotropic murine leukemia virus envelopes were not inhibited by PS vesicles or annexin V. These data indicate that PS is an important cofactor for HIV-1 infection of macrophages.