Cornus officinalis Methanol Extract Upregulates Melanogenesis in Melan-a Cells.
Cornus officinalis Methanol Extract Upregulates Melanogenesis in Melan-a Cells.
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DOI：
10.5487/tr.2015.31.2.165
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发表时间：
2015-06
影响因子：
2.3
通讯作者：
Kim YC
中科院分区：
文献类型：
--
作者：
An YA;Hwang JY;Lee JS;Kim YC
山茱萸广泛分布于韩国，其果实因其滋补、止痛和利尿特性而被韩国、日本和中国用作传统医学的草药。但C.药用植物甲醇提取物（COME）对黑素生成的影响仍然知之甚少。我们评估了COME在melan-a细胞中的黑素生成能力，melan-a细胞是永生化的小鼠黑素细胞。COME以剂量依赖性方式增加黑色素合成。用12.5 μg/mL的COME处理使黑色素含量显著增加36.1%（p < 0.001），甚至高于3-异丁基-1-甲基-黄嘌呤（一种众所周知的色素沉着剂）的水平（31.6%）。COME还上调酪氨酸酶活性及其信使RNA和蛋白质表达。此外，COME上调酪氨酸酶相关蛋白1和2的表达和小眼相关转录因子-M信使RNA的表达。这些结果意味着COME可能适合作为治疗头发变白的天然产品开发。
Cornus officinalis is widely distributed in Korea, and its fruit has been used to make as herbal drug for traditional medicine in Korea, Japan, and China because of its tonic, analgesic, and diuretic properties. However, the effects of C. officinalis methanol extract (COME) on melanogenesis remain poorly understood. We evaluated the melanogenic capability of COME in melan-a cells, which are immortalized mouse melanocytes. COME increased melanin synthesis in a dose-dependent manner. Treatment with 12.5 μg/mL of COME significantly increased melanin content by 36.1% (p < 0.001) to a level even higher than that (31.6%) of 3-isobutyl-1-methyl-xanthine, a well-known pigmentation agent. COME also upregulated tyrosinase activity and its messenger RNA and protein expression. In addition, COME upregulated the expression of tyrosinase-related proteins 1 and 2 and microphthalmia-associated transcription factor-M messenger RNA expression. These results imply that COME may be appropriate for development as a natural product to treat hair graying.