Localization of glycine receptor alpha subunits on bipolar and amacrine cells in primate retina
Localization of glycine receptor alpha subunits on bipolar and amacrine cells in primate retina
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DOI：
10.1002/cne.20555
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发表时间：
2005-07-25
影响因子：
2.5
通讯作者：
Grünert, U
中科院分区：
文献类型：
--
作者：
Jusuf, PR;Haverkamp, S;Grünert, U
视网膜中大约一半的无长突细胞使用主要的抑制性神经递质甘氨酸。无长突细胞为双极、神经节和其他无长突细胞提供突触输出。本研究调查了灵长类动物视网膜中不同类型的双极细胞和无长突细胞在甘氨酸受体(GlyR)亚型表达方面的差异。将针对GlyRα1、α2和α3亚单位的抗体与两极细胞和无长突细胞的免疫组织化学标记相结合，应用于猕猴(Macaca Buncularis)和绒猴(Callithrix Jacchus)视网膜的垂直切片。各亚单位均在内网层表达点状免疫反应。GlyRα2免疫反应阳性斑点密度最高，其次是GlyRα3和GlyRα1 IR斑点。包埋后的电子显微镜显示所有亚单位的突触后位置。双重免疫荧光显示，这三个α亚单位聚集在不同的突触后部位。两种锥体外双极细胞类型(扁平侏儒和弥漫性双极DB3)主要与α1亚基有关。两种双极细胞类型，DB6和杆状双极细胞，主要与α2亚基有关。甘氨酸能的ALL无长突细胞与外侧亚板的α1亚单位突触前，与ON亚板的α2亚单位突触后。另一种可能的甘氨酸能细胞是囊泡型谷氨酸转运体3细胞，它主要与α2亚单位有突触前联系。多巴胺能无长突细胞低密度表达A3亚单位。(C)2005年Wiley-Liss，Inc.
The major inhibitory neurotransmitter glycine is used by about half of the amacrine cells in the retina. Amacrine cells provide synaptic output to bipolar, ganglion, and other amacrine cells. The present study investigated whether different bipolar and amacrine cell types in the primate retina differ with respect to the expression of glycine receptor (GlyR) subtypes. Antibodies specific for the alpha 1, alpha 2, and alpha 3 subunits of the GlyR were combined with immunohistochemical markers for bipolar and amacrine cells and applied to vertical sections of macaque (Macaca fascicularis) and marmoset (Callithrix jacchus) retinae. For all subunits, punctate immunoreactivity was expressed in the inner plexiform layer. The GlyR alpha 2 immunoreactive (IR) puncta occur at the highest density, followed by GlyR alpha 3 and GlyR alpha 1 IR puncta. Postembedding electron microscopy showed the postsynaptic location of all subunits. Double immunofluorescence demonstrated that the three a subunits are clustered at different postsynaptic sites. Two OFF cone bipolar cell types (flat midget and diffuse bipolar DB3), are predominantly associated with the alpha 1 subunit. Two ON bipolar cell types, the DB6 and the rod bipolar cell, are predominantly associated with the alpha 2 subunit. The glycinergic All amacrine cell is presynaptic to the alpha 1 subunit in the OFF-sublamina, and postsynaptic to the alpha 2 subunit in the ON-sublamina. Another putative glycinergic cell, the vesicular glutamate transporter 3 cell, is predominantly presynaptic to the alpha 2 subunit. The dopaminergic amacrine cell expresses the a3 subunit at a low density. (c) 2005 Wiley-Liss, Inc.