ENDOTOXIN AND STAPHYLOCOCCUS-AUREUS ENTEROTOXIN A INDUCE DIFFERENT PATTERNS OF CYTOKINES
ENDOTOXIN AND STAPHYLOCOCCUS-AUREUS ENTEROTOXIN A INDUCE DIFFERENT PATTERNS OF CYTOKINES
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DOI：
10.1016/1043-4666(92)90013-h
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发表时间：
1992-11-01
期刊：
影响因子：
3.8
通讯作者：
ANDERSSON, U
中科院分区：
文献类型：
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作者：
BJORK, L;ANDERSSON, J;ANDERSSON, U
在从健康献血者获得的细胞中，在单细胞水平上评估了金黄色葡萄球菌肠毒素 A (SEA) 和脂多糖 (LPS) 对细胞因子产生的影响。使用细胞因子特异性单克隆抗体染色的固定和透化细胞的紫外显微镜研究细胞因子的产生。评估的细胞因子包括肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1α、IL-1β、IL-6、IL-8、IL-10、IL-2、IL-4、干扰素(IFN)-γ和TNF-β。 LPS 显着产生 IL-1α、IL-1β、TNF-α、IL-6 和 IL-8。 LPS 刺激后，IL-1α、IL-1β、TNF-α 和 IL-8 是主要产物，全部在细胞刺激后 4 小时或之前达到峰值。此外，细胞刺激 12 小时后，IL-10 的产生很明显。在培养物中从未检测到 T 淋巴细胞衍生的细胞因子 TNF-β、IL-2、IFN-γ 和 IL-4。除 IL-8 外，所有细胞因子的产生均在 96 小时时下调。相比之下，在 SEA 刺激的培养物中，主要产物 IL-1α、IL-1β 和 IL-8 的峰值产生发生在 12 小时后。此外，发现 T 淋巴细胞显着产生 TNF-β、TNF-α、IFN-γ 和 IL-2，并在开始后 12-48 小时达到峰值。只有少量的IL-6是明显的。所发现的两种类型的细胞因子模式和动力学可能对应于人类侵袭性革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌感染后的不同临床状况，大肠杆菌感染后疾病的发病速度要快得多。大肠杆菌感染。这些数据还可能意味着，在使用这两种微生物制剂治疗危及生命的疾病时，应考虑不同的免疫调节方法。
The effects ofStaphylococcus aureusenterotoxin A (SEA) and lipopolysaccharide (LPS) in cytokine production were assessed at the single cell level in cells obtained from healthy blood donors. Cytokine production was studied with UV-microscopy of fixed and permeabilized cells stained with cytokine specific monoclonal antibodies. The cytokines evaluated included tumour necrosis factor (TNF)-α, interleukin (IL)-1α, IL-1β, IL-6, IL-8, IL-10, IL-2, IL-4, interferon (IFN)-γ and TNF-β. LPS exhibited marked production of IL-1α, IL-1β, TNF-α, IL-6 and IL-8. After LPS stimulation IL-1α, IL-1β, TNF-α and IL-8 were the dominating products, all peaking at or before 4 hours after cell stimulation. In addition, IL-10 production was evident after 12 hours of cell stimulation. The T-lymphocyte-derived cytokines TNF-β, IL-2, IFN-γ and IL-4 were never detected in the cultures. All cytokine production, except IL-8, was downregulated at 96 hours.In contrast, peak production of IL-1α, IL-1β and IL-8, which were the dominant products, occurred after 12 hours in the SEA-stimulated cultures. Further, a significant T-lymphocyte production of TNF-β, TNF-α, IFN-γ and IL-2 was found with peak production 12–48 hours after initiation. Only low amounts of IL-6 were evident.The two types of cytokine pattern and kinetics found may correspond to the different clinical conditions after invasive Gram-negativeEscherichia colivs Gram-positiveStaphylococcus aureusinfections in humans, with a much more rapid onset of disease afterE. coliinfections. The data may also imply that different immunomodulating approaches should be considered in life-threatening diseases with the two microbacterial agents.