COLLAPSIN-INDUCED GROWTH CONE COLLAPSE MEDIATED BY AN INTRACELLULAR PROTEIN RELATED TO UNC-33
COLLAPSIN-INDUCED GROWTH CONE COLLAPSE MEDIATED BY AN INTRACELLULAR PROTEIN RELATED TO UNC-33
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DOI：
10.1038/376509a0
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发表时间：
1995-08-10
期刊：
影响因子：
64.8
通讯作者：
STRITTMATTER, SM
中科院分区：
文献类型：
--
作者：
GOSHIMA, Y;NAKAMURA, F;STRITTMATTER, SM
新认识的信号素家族（2-4）的成员Collapsin（1）通过抑制生长锥扩展（1-5），在神经发育过程中有助于轴突探路。崩溃作用的机制知之甚少，在这里，我们使用爪蟾Laevis卵母细胞表达系统来识别参与折叠信号传导的分子，因为多个实验增加了异源三聚体GTP结合蛋白可能参与这些事件的可能性（6-9）。分离了相对分子质量（M（r））62K（CRMP-62）的折叠蛋白反应介质蛋白，以分离出collapsin诱导的X. laevis卵母细胞中的内向电流。 CRMP-62与unc-33共享同源性，这是适当定向的轴突延伸所需的线虫神经元蛋白（10-12），CRMP-62仅在发育中的鸡神经系统中定位。将抗CRMP-62抗体引入背根神经神经元中阻断崩溃的生长锥塌陷。 CRMP-62似乎是由身份不明的跨膜倒塌蛋白引发的信号级联的细胞内成分。
COLLAPSIN(1), a member of the newly recognized semaphorin family(2-4), contributes to axonal pathfinding during neural development by inhibiting growth cone extension(1-5). The mechanism of collapsin action is poorly understood, Here we use a Xenopus laevis oocyte expression system to identify molecules involved in collapsin signalling, because several experiments have raised the possibility that heterotrimeric GTP-binding proteins might participate in these events(6-9). A collapsin response mediator protein of relative molecular mass (M(r)) 62K (CRMP-62) required for collapsin-induced inward currents in X. laevis oocytes is isolated. CRMP-62 shares homology with UNC-33, a nematode neuronal protein required for appropriately directed axonal extension(10-12), CRMP-62 is localized exclusively in the developing chick nervous system. Introduction of anti-CRMP-62 antibodies into dorsal root ganglion neurons blocks collapsin-induced growth cone collapse. CRMP-62 appears to be an intracellular component of a signalling cascade initiated by an unidentified transmembrane collapsin-binding protein.