Transcriptomic responses of Nile tilapia (Oreochromis niloticus) liver to environmental concentration of di(2-ethylhexyl)phthalate
Transcriptomic responses of Nile tilapia (Oreochromis niloticus) liver to environmental concentration of di(2-ethylhexyl)phthalate
复制标题
尼罗罗非鱼（Oreochromis niloticus）肝脏对环境浓度邻苯二甲酸二（2-乙基己基）酯的转录组反应
DOI：
10.1016/j.ecoenv.2018.08.100
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发表时间：
2018
影响因子：
6.8
通讯作者：
Cai Wengui
中科院分区：
文献类型：
--
作者：
Zhang Linbao;Hu Ying;Sun Wei;Chen Si;Jia Xiaoping;Cai Wengui
邻苯二甲酸二（2-乙基己基）酯（DEHP）是目前检测最多的邻苯二甲酸酯类化合物，对水生生物具有多种毒性。为了了解鱼类对DEHP的分子反应，我们对暴露于环境浓度DEHP的罗非鱼（Oreochromis niloticus）肝脏进行了转录组学分析。分别从对照和DEHP处理的文库中检索到总计30.10 Mb和30.16 Mb的干净读段。所有干净读段的从头组装获得58，585个单基因。在比较两个文库后，分别鉴定出2814个和1790个基因显著增加和降低。基因本体论（GO）分类系统和京都基因与基因组百科全书（KEGG）数据库分析表明，DEHP显著干扰了免疫、内分泌和生殖系统、脂质代谢等相关基因的表达水平，采用定量实时PCR验证了RNA测序（RNA-seq）分析结果。由此产生的数据为探索罗非鱼对DEHP暴露反应的分子基础提供了新的见解。
Di-(2-ethylhexyl) phthalate (DEHP) is currently the most frequently detected phthalic acid esters (PAEs) compounds and can induce diverse toxicities on aquatic organisms. To understand the molecular responses of fish to DEHP, we performed transcriptomic profiles in liver of tilapia (Oreochromis niloticus) which were exposed to environmental concentration of DEHP. A total of 30.10 Mb and 30.16 Mb clean reads were retrieved from the control and DEHP treated libraries, respectively. De-novo assembly of all the clean reads obtained 58,585 unigenes. After comparing the two libraries, 2814 and 1790 genes were identified as significantly increased and depressed, respectively. Gene ontology (GO) classification system and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis demonstrated that DEHP significantly disturbed the expression level of genes associated with immunity, endocrine and reproductive system, lipid metabolism and so on. Quantitative realtime PCR was performed to validate the results of RNA-sequencing (RNA-seq) analysis. The resulting data provide new insights for exploring the molecular basis of tilapia response to DEHP exposure.