INVIVO DEMONSTRATION OF INSULIN-RECEPTOR DEFECT WITH I-123 LABELED INSULIN AND SCINTIGRAPHIC SCANNING IN SEVERE INSULIN RESISTANCE
INVIVO DEMONSTRATION OF INSULIN-RECEPTOR DEFECT WITH I-123 LABELED INSULIN AND SCINTIGRAPHIC SCANNING IN SEVERE INSULIN RESISTANCE
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DOI：
10.2337/diacare.15.5.651
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发表时间：
1992-05-01
期刊：
影响因子：
16.2
通讯作者：
SODOYEZ, JC
中科院分区：
文献类型：
--
作者：
DOZIO, N;MICOSSI, P;SODOYEZ, JC
目的：人类胰岛素受体功能的研究通常是在体外利用容易获得的细胞，如红细胞和成纤维细胞。尽管这些细胞在代谢上不是胰岛素作用的重要靶点，但从它们获得的信息通常被视为其他组织的代表。本研究的目的是在体外和体内研究胰岛素的主要靶器官之一肝脏上的胰岛素受体。研究设计和方法-确定了一名患有严重胰岛素抵抗的16岁女孩。对患者和6名非糖尿病志愿者的红细胞胰岛素受体结合进行了检测。用核素扫描技术研究了I-123标记的胰岛素在胰岛素抵抗患者和10名非糖尿病志愿者体内的生物分布。结果该患者的红细胞[I-125]胰岛素结合率显著降低。在体内，I-123-胰岛素的生物分布特征是肝脏缺乏激素摄取(对照组为注射剂量的4%)，而肾脏则有强烈的放射性蓄积。结论--我们的研究表明，我们的研究表明，用一种低放射毒性的非侵入性技术，可以在体内直接显示胰岛素与肝脏受体结合的缺陷。
OBJECTIVE - Insulin-receptor function in humans is usually studied in vitro on readily available celts, e.g., erythrocytes and fibroblasts. Although these cells are not metabolically important targets for insulin action, information derived from them are often taken as representative of other tissues. The aim of this study was to investigate insulin receptors in vitro on erythrocytes and in vivo on one of the main insulin-target organs, the liver.RESEARCH DESIGN AND METHODS - A 16-yr-old girl affected by severe insulin resistance was identified. Insulin receptor binding was measured on the erythrocytes of the patient and of 6 nondiabetic volunteers. The biodistribution of I-123-labeled insulin was studied in vivo by scintigraphic scanning in the insulin-resistant patient and in 10 nondiabetic volunteers.RESULTS - Erythrocytes of this patient displayed a markedly reduced [I-125]insulin binding. In vivo I-123-insulin biodistribution was characterized by lack of hormone uptake by the liver (4 vs. 21% of the injected dose in control subjects) contrasting with intense accumulation of radioactivity in the kidneys.CONCLUSIONS - Our studies show that defects of insulin binding can be directly demonstrated in vivo on liver receptors with a noninvasive technique with low radiotoxicity.