Role of hemicellulose removal during dilute acid pretreatment on the cellulose accessibility and enzymatic hydrolysis of compositionally diverse sugarcane hybrids
Role of hemicellulose removal during dilute acid pretreatment on the cellulose accessibility and enzymatic hydrolysis of compositionally diverse sugarcane hybrids
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DOI：
10.1016/j.indcrop.2017.11.053
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发表时间：
2018-01-01
影响因子：
5.9
通讯作者：
Ferraz, Andre
中科院分区：
文献类型：
--
作者：
de Oliveira Santos, Victor Tabosa;Siqueira, Germano;Ferraz, Andre
用稀硫酸预处理三种不同的甘蔗杂交种，以评估半纤维素去除对葡聚糖酶促转化效率的作用。随着反应间隔的增加，预处理的主要效果沿着是选择性去除半纤维素，这将葡聚糖酶促水解的效率提高到63%转化率。具有最低初始木质素含量的杂种表现出最高的酶转化效率。半纤维素去除后，预处理材料的细胞壁孔隙度降低，这表明木质素重新分布到细胞壁中。酸预处理样品的后脱木质化将酶促葡聚糖转化率提高到92- 100%。基于Simons的差异染色技术估计葡聚糖的可及表面，该技术与酶促葡聚糖转化效率和预处理样品的固有样品参数相关，我们将其命名为“可用葡聚糖”。可用葡聚糖定义为样品中葡聚糖含量与非葡聚糖组分总和的比率。酶促葡聚糖转化水平与可用的葡聚糖值适当吻合。
Three diverse sugarcane hybrids were pretreated with dilute sulfuric acid to evaluate the role of hemicellulose removal on the efficiency of enzymatic conversion of glucan. The main pretreatment effect along with increasing reaction intervals was the selective removal of hemicellulose, which enhanced the efficiency of glucan enzymatic hydrolysis up to 63% conversion. The hybrid with the lowest initial lignin content showed the highest enzymatic conversion efficiency. Cell wall porosity of pretreated materials decreased after hemicellulose removal, which suggests lignin redistribution into the cell walls. Post-delignification of acid-pretreated samples enhanced enzymatic glucan conversions to 92-100%. The accessible surface of glucan was estimated based on Simons' differential stain technique, which correlated with enzymatic glucan conversion efficiencies and with an intrinsic sample parameter of pretreated samples that we named "available glucan". Available glucan was defined as the ratio of glucan content to the sum of non-glucan components of the samples. Enzymatic glucan conversion levels fit properly with available glucan values.