A novel G-protein-coupled receptor gene expressed in striatum
A novel G-protein-coupled receptor gene expressed in striatum
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DOI：
10.1006/geno.2000.6340
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发表时间：
2000-11-01
期刊：
影响因子：
4.4
通讯作者：
Ito, T
中科院分区：
文献类型：
--
作者：
Mizushima, K;Miyamoto, Y;Ito, T
在大鼠脑内区域特异性转录物的差异显示筛选中发现了一个新的纹状体特异性转录物，编码一个孤儿G蛋白偶联受体(GPCR)，命名为STRG/GPR88，我们从人(HGMW批准的符号GPR88)和小鼠中分离出它的同源物，并将它们分别定位在染色体1p21.3和3G1上。STRG/Gpr88蛋白的一级序列在人和啮齿类动物之间高度保守，与生物胺受体的同源性最高，然而，Strg/Gpr88缺乏一些在所有已知的生物胺受体中保守的残基，因此可能代表了一种新的GPCR亚型。Northern印迹和原位杂交分析表明，Strg/Gpr88转录本在人和啮齿类动物中几乎只在纹状体表达。初级结构的显著保守和独特的表达模式可能表明Strg/Gpr88在纹状体的基本功能中发挥作用，如控制运动行为，(C)2000学术出版社。
Differential display screening for region-specific transcripts in rat brain revealed a novel striatum-specific transcript encoding an orphan G-protein-coupled receptor (GPCR) designated Strg/Gpr88 for striatum-specific GPCR, We isolated its homologues from human (HGMW-approved symbol GPR88) and mouse and mapped them to chromosomes 1p21.3 and 3G1, respectively. These loci are syntenic to each other, thereby suggesting their orthology, The predicted primary sequences of Strg/Gpr88 proteins are highly conserved between human and rodents and show the highest level of homology to receptors for biogenic amines, However, Strg/Gpr88 lacks some residues conserved in all known biogenic amine receptors and hence may represent a novel subtype of GPCR. Northern blot and in situ hybridization analyses revealed that Strg/Gpr88 transcripts are expressed almost exclusively in striatum in both human and rodents. Remarkable conservation in primary structure and a unique expression pattern may indicate a role for Strg/Gpr88 in the fundamental functions of striatum such as the control of motor behavior, (C) 2000 Academic Press.