Increased airway reactivity in a neonatal mouse model of continuous positive airway pressure
Increased airway reactivity in a neonatal mouse model of continuous positive airway pressure
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DOI：
10.1038/pr.2015.90
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发表时间：
2015-08-01
影响因子：
3.6
通讯作者：
MacFarlane, Peter M.
中科院分区：
文献类型：
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作者：
Mayer, Catherine A.;Martin, Richard J.;MacFarlane, Peter M.
背景：持续气道正压通气(CPAP)是早产儿重症监护中应用的一种主要的呼吸支持形式，但其对气道功能的长期影响尚不清楚。方法：我们建立了CPAP治疗的新生小鼠模型，以确定其是否改变晚期AW的反应性。未麻醉的自发呼吸小鼠戴上口罩，从出生后第1天开始连续7天给予CPAP(6 cmH(2)O，3h/d)。用体外活肺切片制片法测定AW对乙酰甲胆碱的反应性。结果：与未经处理的对照组相比，1周的CPAP增加了雄性小鼠对乙酰甲胆碱的AW反应性，但对雌性小鼠的AW反应性没有影响。CPAP治疗结束后，雄性小鼠AW高反应性持续2wk(P21)。然而，四天的CPAP并没有显著增加AW的反应性。雌性在P21时也表现出AW高反应性，提示对早期(7d)CPAP治疗有延迟反应。7d的CPAP对乙酰甲胆碱高反应性的影响仅限于较小的AWS，而较大的AWS相对不受影响。结论：这些数据可能对我们理解新生儿CPAP治疗在早产儿重症监护中的潜在长期后果有重要意义。
BACKGROUND: Continuous positive airway pressure (CPAP) is a primary form of respiratory support used in the intensive care of preterm infants, but its long-term effects on airway (AW) function are unknown.METHODS: We developed a neonatal mouse model of CPAP treatment to determine whether it modifies later AW reactivity. Unanesthetized spontaneously breathing mice were fitted with a mask to deliver CPAP (6 cmH(2)O, 3 h/day) for 7 consecutive days starting at postnatal day 1. AW reactivity to methacholine was assessed using the in vitro living lung slice preparation.RESULTS: One week of CPAP increased AW responsiveness to methacholine in male, but not female mice, compared to untreated control animals. The AW hyper-reactivity of male mice persisted for 2 wk (at P21) after CPAP treatment ended. Four days of CPAP, however, did not significantly increase AW reactivity. Females also exhibited AW hyper-reactivity at P21, suggesting a delayed response to early (7 d) CPAP treatment. The effects of 7 d of CPAP on hyper-reactivity to methacholine were unique to smaller AWs whereas larger ones were relatively unaffected.CONCLUSION: These data may be important to our understanding of the potential long-term consequences of neonatal CPAP therapy used in the intensive care of preterm infants.