Hypoxia enhances colony formation and proliferation but inhibits differentiation of human dental pulp cells
Hypoxia enhances colony formation and proliferation but inhibits differentiation of human dental pulp cells
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DOI：
10.1016/j.archoralbio.2010.06.005
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发表时间：
2010-09-01
影响因子：
3
通讯作者：
Tezuka, Ken-ichi
中科院分区：
文献类型：
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作者：
Iida, Kazuki;Takeda-Kawaguchi, Tomoko;Tezuka, Ken-ichi
由于人牙髓细胞（human dental pulp cells，hDPC）在体内处于低氧环境中，因此低氧条件适合于hDPC的建立和维持。因此，我们目前在体外研究了缺氧对hDPC增殖和分化的影响。在3%O-2下生长的hDPC显示出显著高于在21%O-2下生长的hDPC的增殖率。然后，我们从患有炎症的老年患者的牙齿制备了hDPC的缺氧培养物，并成功地恢复和扩增了少量的hDPC。这些细胞被证实具有成骨/成牙分化的能力。缺氧抑制hDPC在体外的成骨/牙向分化，并增加表达STRO-1，一种早期间充质干细胞标记物的细胞数量。这种简单的方法将增加从受损和/或老化的组织获得活的hDPC的可能性，通常难以从受损和/或老化的组织分离具有分化能力的活干细胞。(C)2010爱思唯尔有限公司保留所有权利。
The hypoxia condition was expected to be suitable for the establishment and maintenance of human dental pulp cells (hDPCs), because they reside in a low-oxygen environment in vivo. Therefore, we presently examined the effects of hypoxia on the proliferation and differentiation of hDPCs in vitro. hDPCs grown under 3% O-2 showed a significantly higher proliferation rate than those under 21% O-2. Then, we prepared hypoxic cultures of hDPCs from older patients' teeth having inflammation and succeeded in recovering and expanding a small number of hDPCs. These cells were confirmed to have capability for osteo/odontogenic differentiation. Hypoxia suppressed the osteo/odontogenic differentiation of hDPCs in vitro and increased the number of cells expressing STRO-1, an early mesenchymal stem cell marker. This simple method will increase the possibility to obtain living hDPCs from damaged and/or aged tissues, from which it is ordinarily difficult to isolate living stem cells with differentiation capability. (C) 2010 Elsevier Ltd. All rights reserved.