A magnetic resonance tuning sensor for the MRI detection of biological targets
A magnetic resonance tuning sensor for the MRI detection of biological targets
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DOI：
10.1038/s41596-018-0057-y
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发表时间：
2018-11-01
期刊：
影响因子：
14.8
通讯作者：
Cheon, Jinwoo
中科院分区：
文献类型：
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作者：
Shin, Tae-Hyun;Kang, Sunghwi;Cheon, Jinwoo
检测生物体中感兴趣的特定分子的传感器可以成为研究生物功能和疾病的有用工具。在这里，我们提供了一个协议的纳米传感器，可以非侵入性地检测生物学上重要的目标与磁共振成像（MRI）的建设。这些传感器的关键工作原理是磁共振调谐（MRET），这是一种发生在超顺磁性淬灭剂和顺磁性增强剂之间的距离依赖现象。两个磁分量之间的距离的变化调制增强器的纵向（T-1）弛豫率。在该MRET传感器中，距离变化通过相互作用的接头实现，这些接头在与靶分子相互作用时进行结合、切割或折叠/解折叠。通过模块化结合合适的连接体，MRET传感器可应用于广泛的目标。我们展示了用于酶，核酸序列和pH的MRET传感器的三个例子。该协议包括三个阶段：（i）化学合成和淬灭剂的表面修饰，（ii）与相互作用的连接子和增强子的缀合，以及（iii）生物靶标的MRI传感。整个过程需要3D。
Sensors that detect specific molecules of interest in a living organism can be useful tools for studying biological functions and diseases. Here, we provide a protocol for the construction of nanosensors that can non-invasively detect biologically important targets with magnetic resonance imaging (MRI). The key operating principle of these sensors is magnetic resonance tuning (MRET), a distance-dependent phenomenon occurring between a superparamagnetic quencher and a paramagnetic enhancer. The change in distance between the two magnetic components modulates the longitudinal (T-1) relaxivity of the enhancer. In this MRET sensor, distance variation is achieved by interactive linkers that undergo binding, cleavage, or folding/unfolding upon their interaction with target molecules. By the modular incorporation of suitable linkers, the MRET sensor can be applied to a wide range of targets. We showcase three examples of MRET sensors for enzymes, nucleic acid sequences, and pH. This protocol comprises three stages: (i) chemical synthesis and surface modification of the quencher, (ii) conjugation with interactive linkers and enhancers, and (iii) MRI sensing of biological targets. The entire procedure takes up to 3 d.