Treatment with the MAPK kinase inhibitor U0126 during the first two hours of in vitro maturation improves bovine oocyte developmental competence.
Treatment with the MAPK kinase inhibitor U0126 during the first two hours of in vitro maturation improves bovine oocyte developmental competence.
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在体外成熟的前两个小时内使用 MAPK 激酶抑制剂 U0126 进行治疗可改善牛卵母细胞的发育能力。
DOI：
10.1111/rda.13096
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
H. Tamada.
中科院分区：
文献类型：
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作者：
T. Shinohara;Y. Ohta;N. Kawate;M. Takahashi;N. Sakagami;T. Inaba;H. Tamada.
本研究检测了在牛体外成熟的前2小时内用U0126处理对牛发育能力和卵母细胞与卵丘细胞之间的间隙连接（GAPJ）通讯的影响。5 μM U0126处理组卵母细胞发育至囊胚期的百分率（28%）显著高于对照组（15%，p <0.05），而10 μM U0126处理组卵母细胞发育至囊胚期的百分率（18%）则无显著差异。与对照组相比，用5 μM U0126处理的组中GAPJ的分解延迟，如通过连接蛋白43的免疫组织化学检查所估计的，连接蛋白43是GAPJ的主要成分。这些结果表明，在体外成熟过程中用5 μM U0126处理可延迟GAPJ分解并提高牛卵母细胞的发育能力。
ContentsThis study examined the effects of treatment with U0126, which inhibits MAPK by inhibiting MAPK kinase, during the first 2 hr of in vitro maturation on bovine developmental competence and on gap junction (GAPJ) communication between the oocyte and cumulus cells. The percentage of oocytes developing to the blastocyst stage in the group treated with 5 μM U0126 (28%) was significantly higher than that in controls (15%,p<.05), while that in the group treated with 10 μM U0126 (18%) was not. Breakdown of the GAPJs was delayed in the group treated with 5 μM U0126 when compared to controls, as estimated by immunohistochemical examination of connexin 43, which is a primary constituent of the GAPJs. These results indicate that treatment with 5 μM U0126 during in vitro maturation delays GAPJ breakdown and improves bovine oocyte developmental competence.