Recombinant vesicular stomatitis virus expressing respiratory syncytial virus (RSV) glycoproteins: RSV fusion protein can mediate infection and cell fusion
Recombinant vesicular stomatitis virus expressing respiratory syncytial virus (RSV) glycoproteins: RSV fusion protein can mediate infection and cell fusion
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DOI：
10.1006/viro.1998.9535
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发表时间：
1999-02-01
期刊：
影响因子：
3.7
通讯作者：
Rose, JK
中科院分区：
文献类型：
--
作者：
Kahn, JS;Schnell, MJ;Rose, JK
编码呼吸道合胞病毒（RSV）附着（G）和融合（F）包膜糖蛋白的基因分别作为重组水泡性口炎病毒（VSV）中的额外基因表达。用VSV-RSV F重组体感染的细胞形成大的合胞体，说明在不存在其它RSV蛋白的情况下F的融合活性。F和G糖蛋白都在细胞表面表达并掺入病毒体中。这些蛋白的掺入不需要VSV G的胞质尾序列。使用一种化合物，氯化铵，提高内体pH值，我们表明，RSV F糖蛋白的存在下，重组VSV的包膜允许通过低pH值的非依赖性途径的感染性。表达RSV糖蛋白的重组VSV可用作RSV疫苗。(C)北京：科学出版社.
The genes encoding the respiratory syncytial virus (RSV) attachment (G) and fusion (F) envelope glycoproteins were expressed separately as additional genes in recombinant vesicular stomatitis viruses (VSV). Cells infected with the VSV-RSV F recombinant formed large syncytia illustrating the fusion activity of F in absence of other RSV proteins. Both F and G glycoproteins were expressed at the cell surface and incorporated into virions. Incorporation of these proteins did not require cytoplasmic tail sequences of VSV G. Using a compound, ammonium chloride, that raises the endosomal pH, we showed that presence of the RSV F glycoprotein in the envelope of recombinant VSV allowed for infectivity through a low-pH-independent pathway. Recombinant VSV expressing RSV glycoproteins could be useful as an RSV vaccine. (C) 1999 Academic Press.