Genistein inhibits growth of human uveal melanoma cells and affects microRNA-27a and target gene expression.
Genistein inhibits growth of human uveal melanoma cells and affects microRNA-27a and target gene expression.
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DOI：
10.3892/or_00000472
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发表时间：
2009-09
期刊：
影响因子：
4.2
通讯作者：
Qingmin Sun;Rihong Cong;Hongli Yan;Haijuan Gu;Ying Zeng;Nannan Liu;Jie Chen;Bin Wang
中科院分区：
文献类型：
--
作者：
Qingmin Sun;Rihong Cong;Hongli Yan;Haijuan Gu;Ying Zeng;Nannan Liu;Jie Chen;Bin Wang
染料木黄酮是一种从大豆中分离出的异黄酮，已被发现是一种有效的抗肿瘤剂。然而，染料木黄酮对人葡萄膜黑色素瘤细胞的作用和染料木黄酮抑制肿瘤发生的确切机制仍不清楚。在本研究中，我们探讨了染料木黄酮抑制人葡萄膜黑色素瘤细胞生长的可能活性，并研究了染料木黄酮对microRNA-27 a（miR-27 a）及其靶基因锌指和BTB结构域10（ZBTB 10）表达水平的可能作用。结果表明，葡萄膜黑色素瘤细胞生长的显着抑制在时间和剂量相关的方式。体内实验也表明染料木黄酮处理组能明显抑制移植瘤的生长。进一步的功能分析显示，miR-27 a及其靶基因ZBTB 10的水平基于染料木黄酮的剂量而显著不同。总之，本研究表明，金雀异黄素对人葡萄膜黑色素瘤细胞具有生长抑制活性。此外，我们首次报道了染料木黄酮的抗肿瘤活性与miR-27 a介导的调控机制之间的相关性。
Genistein, an isoflavone isolated from soybean, has been found to be a potent antitumor agent. However, both the effect of genistein on human uveal melanoma cells and the precise mechanism by which genistein suppresses tumorigenesis remain unclear. In the present study, we explored the possible activity of genistein to inhibit human uveal melanoma cell growth and investigated the possible role of genistein on microRNA-27a (miR-27a) as well as its target gene zinc finger and BTB domain containing 10 (ZBTB10) expression levels. The results suggested a significant inhibition of uveal melanoma cell growth in a time- and dose-related manner. In vivo study also indicated treatment groups with genistein could significantly inhibit the growth of xenografts. Further functional assays revealed that the levels of miR-27a and its target gene ZBTB10 were significantly different based on the dose of genistein. In conclusion, the present study demonstrates that genistein exerts growth inhibitory activities in human uveal melanoma cells. Moreover, we for the first time report a correlation between antitumor activity of genistein and miR-27a mediated regulatory mechanism.