The method controls the story - Sampling method impacts on the detection of pore-water nitrogen concentrations in streambeds.

The method controls the story - Sampling method impacts on the detection of pore-water nitrogen concentrations in streambeds.
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该方法控制了故事 - 采样方法对河床孔隙水氮浓度检测的影响。

DOI:
10.1016/j.scitotenv.2019.136075
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发表时间:
2020
期刊:
The Science of the total environment
影响因子:
--
通讯作者:
Comer-Warner S
Comer-Warner S
中科院分区:
--
文献类型:
--
作者:
Comer-Warner S

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河床中的生物地球化学梯度很陡,在短距离内可能会发生变化,这往往使沉积物生物地球化学过程的充分表征具有挑战性。本文提供了一个概述和比较的河床孔隙水采样方法,突出他们的能力,以解决我们的理解的河床地球化学过程的差距。这项工作的审查和评论可用的孔隙水采样技术,以监测河床的地球化学条件,包括其特征的空间和时间分辨率,以及相关的优点和局限性。实地研究比较三种常用的孔隙水采样技术(多级微型测压仪,微型驱动点采样器和薄膜凝胶中的扩散平衡)进行了评估所观察到的硝酸盐和铵浓度分布的差异。孔隙水硝酸盐浓度没有显着差异采样方法(p值= 0.54)与平均浓度为2.53,4.08和4.02毫克l− 1观察到的多级微型压力计,微型驱动点采样器和薄膜凝胶采样器中的扩散平衡,分别。然而,孔隙水铵浓度在多级微型测压仪提取的孔隙水中显著较高(3.83 mg l−1),而在微型驱动点采样器采样的孔隙水中显著较低(1.05 mg l−1,p值<0.01)。在实验室条件下,进一步探讨了所观察到的孔隙水铵浓度分布之间的差异主动(吸力:多级微型测压仪)和被动(平衡;扩散平衡薄膜凝胶)采样器。当在薄膜凝胶中通过扩散平衡采样时,测量的孔隙水铵浓度显著高于多级微型测压仪(所有p值≤0.02)。本研究的结果对解释基于现场的潜流带地球化学循环研究具有重要意义,并强调需要对采样协议进行更系统的测试。第一次,不同的主动和被动孔隙水采样方法的影响在这里系统地解决,突出孔隙水采样方法的选择在何种程度上影响研究成果,与以前发表的工作以及未来的研究解释相关。
Biogeochemical gradients in streambeds are steep and can vary over short distances often making adequate characterisation of sediment biogeochemical processes challenging. This paper provides an overview and comparison of streambed pore-water sampling methods, highlighting their capacity to address gaps in our understanding of streambed biogeochemical processes. This work reviews and critiques available pore-water sampling techniques to characterise streambed biogeochemical conditions, including their characteristic spatial and temporal resolutions, and associated advantages and limitations. A field study comparing three commonly-used pore-water sampling techniques (multilevel mini-piezometers, miniature drivepoint samplers and diffusive equilibrium in thin-film gels) was conducted to assess differences in observed nitrate and ammonium concentration profiles. Pore-water nitrate concentrations did not differ significantly between sampling methods (p-value = 0.54) with mean concentrations of 2.53, 4.08 and 4.02 mg l−1observed with the multilevel mini-piezometers, miniature drivepoint samplers and diffusive equilibrium in thin-film gel samplers, respectively. Pore-water ammonium concentrations, however, were significantly higher in pore-water extracted by multilevel mini-piezometers (3.83 mg l−1) and significantly lower where sampled with miniature drivepoint samplers (1.05 mg l−1,p-values <0.01). Differences in observed pore-water ammonium concentration profiles between active (suction: multilevel mini-piezometers) and passive (equilibrium; diffusive equilibrium in thin-film gels) samplers were further explored under laboratory conditions. Measured pore-water ammonium concentrations were significantly greater when sampled by diffusive equilibrium in thin-film gels than with multilevel mini-piezometers (allp-values ≤0.02).The findings of this study have critical implications for the interpretation of field-based research on hyporheic zone biogeochemical cycling and highlight the need for more systematic testing of sampling protocols. For the first time, the impact of different active and passive pore-water sampling methods is addressed systematically here, highlighting to what degree the choice of pore-water sampling methods affects research outcomes, with relevance for the interpretation of previously published work as well as future studies.
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