Holographic opto-fluidic microscopy.
Holographic opto-fluidic microscopy.
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DOI：
10.1364/oe.18.027499
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发表时间：
2010-12-20
期刊：
影响因子：
3.8
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Bishara W;Zhu H;Ozcan A
在过去的十年中，微流体已经创建了一个多功能平台，通过提高分析的成本，紧凑性和吞吐量方面，显着推进了微尺度生物和物体的控制，处理和研究方式。微流控也扩展到光学领域，以创建可重构和灵活的光学器件，如可重构透镜、激光器、波导、开关和片上显微镜。本文提出了一种基于无透镜全息成像的新型光学流体显微镜技术，即全息光学流体显微镜（homm）。这种成像方式补充了微流体提供的小型化，并允许将显微镜集成到现有的具有各种功能的片上微流体设备中。我们的成像方式利用部分相干的在线全息和像素超分辨率来创建在微流体通道内流动的物体的高分辨率振幅和相位图像，我们通过对秀丽隐杆线虫、贾第鞭毛虫和桑树花粉的成像来证明这一点。HOM不涉及复杂的制造工艺或精确的对准，也不需要在微流体通道内高度均匀的物体流动。
Over the last decade microfluidics has created a versatile platform that has significantly advanced the ways in which micro-scale organisms and objects are controlled, processed and investigated, by improving the cost, compactness and throughput aspects of analysis. Microfluidics has also expanded into optics to create reconfigurable and flexible optical devices such as reconfigurable lenses, lasers, waveguides, switches, and on-chip microscopes. Here we present a new opto-fluidic microscopy modality, i.e., Holographic Opto-fluidic Microscopy (HOM), based on lensless holographic imaging. This imaging modality complements the miniaturization provided by microfluidics and would allow the integration of microscopy into existing on-chip microfluidic devices with various functionalities. Our imaging modality utilizes partially coherent in-line holography and pixel super-resolution to create high-resolution amplitude and phase images of the objects flowing within micro-fluidic channels, which we demonstrate by imaging C. elegans, Giardia lamblia, and Mulberry pollen. HOM does not involve complicated fabrication processes or precise alignment, nor does it require a highly uniform flow of objects within microfluidic channels.