Detection and serotyping of human adenoviruses from patients with influenza-like illness in mongolia.
Detection and serotyping of human adenoviruses from patients with influenza-like illness in mongolia.
复制标题
蒙古流感样疾病患者人类腺病毒的检测和血清分型。
DOI：
--
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
P. Nymadawa
中科院分区：
文献类型：
--
作者：
K. Tohma;Namuuntsetsegiin Bayasgalan;A. Suzuki;B. Darma;H. Oshitani;P. Nymadawa
人类腺病毒（HAdV）是造成约5%-10%的急性呼吸道感染的原因。在亚洲国家，常见的呼吸道HAdV血清型多种多样。然而，在蒙古国没有关于HAdV血清型流行的充分报道。在2010年1月至2011年5月期间，对1,950份流感样疾病患者的流感阴性样本（包括有眼部症状患者的眼拭子）进行了HAdV筛查，40份样本（2.1%）为HAdV阳性。在这40个样品中，31个样品是阳性的六邻体基因用于系统发育分析，通过PCR测定。我们鉴定了7种不同的血清型。我们构建了HAdV-B7和HAdV-B3的系统发育树，这是本研究中最常见的2种血清型。所有检测到的HAdV-B7和-B3蒙古株序列完全相同。已知与流行性角膜结膜炎（EKC）相关的HAdV-D8从鼻咽和眼拭子中检测到。蒙古株和引起EKC的菌株中可能影响组织嗜性的六邻体和纤维基因的氨基酸序列之间没有差异。
Human adenoviruses (HAdVs) are responsible for approximately 5%-10% of acute respiratory infections. The serotypes of commonly detected respiratory HAdV in Asian countries are diverse. However, there are no well-documented reports of circulating HAdV serotypes in Mongolia. Between January 2010 and May 2011, 1,950 influenza-negative samples from patients with influenza-like illness, including eye swabs from patients with eye symptoms, were screened for HAdV, and 40 samples (2.1%) were positive for HAdVs. Among these 40 samples, 31 samples were positive for the hexon gene used in phylogenetic analysis, as determined by PCR. We identified 7 different serotypes. We constructed the phylogenetic trees of HAdV-B7 and HAdV-B3, the 2 most commonly detected serotypes in this study. All detected HAdV-B7 and -B3 Mongolian strains had identical sequences. HAdV-D8, known to be associated with epidemic keratoconjunctivitis (EKC), was detected from nasopharyngeal and eye swabs. There was no difference between the amino acid sequences of the hexon and fiber genes that may affect tissue tropism in Mongolian strains and those in EKC-causing strains.
影响因子：
3.7
作者：
Robinson CM;Singh G;Henquell C;Walsh MP;Peigue-Lafeuille H;Seto D;Jones MS;Dyer DW;Chodosh J
通讯作者：
Chodosh J