Unusual Nanofractal Microparticles for Rapid Protein Capture and Release.
Unusual Nanofractal Microparticles for Rapid Protein Capture and Release.
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用于快速蛋白质捕获和释放的不寻常的纳米分形微粒。
DOI：
10.1002/smll.202102802
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发表时间：
2021-07
期刊：
影响因子：
13.3
通讯作者：
Wang Shutao
中科院分区：
文献类型：
--
作者：
Song Yongyang;Dong Xuefang;Shang Danyi;Zhang Xiaofei;Li Xiuling;Liang Xinmiao;Wang Shutao
离子交换多孔微粒广泛应用于蛋白质分离，但其完全多孔的结构往往导致扩散速率慢和分离时间长。在这里不寻常的纳米分形微粒合成的静电相互作用调节乳液界面聚合的策略，表现出优异的快速蛋白质捕获，释放和分离的能力。纳米分形微粒表面纳米结构的生长可以通过改变离子基团之间的静电排斥由弱到强来控制。纳米分形微粒提供了离子基团和蛋白质之间的3D接触模型，在初始捕获和释放阶段实现了快速的蛋白质扩散速率，并实现了类似大小的蛋白质的快速有效分离，作为概念验证，上级多孔微粒。这一策略为生物医学、环境和食品等各个领域的微粒合成提供了一种有效和通用的方法，以实现快速有效的分离。
Ion exchange porous microparticles are widely used for protein separation, but their totally porous structure often leads to slow diffusion rate and long separation time. Here unusual nanofractal microparticles synthesized by a strategy of electrostatic interaction regulated emulsion interfacial polymerization are demonstrated that exhibit excellent capability of rapid protein capture, release, and separation. The growth of nanostructures at nanofractal microparticle surface can be controlled by changing electrostatic repulsion between ion groups from weak to strong. The nanofractal microparticles provide a 3D contact model between ion groups and proteins, enable fast protein diffusion rate at initial capture and release stage, and realize rapid and efficient separation of similarly sized proteins as a proof of concept, superior to porous microparticles. This strategy offers an effective and general way for the synthesis of microparticles towards rapid and efficient separation in various fields of biomedicine, environment, and food.