Effect of cadmium stress on inductive enzymatic and nonenzymatic responses of ROS and sugar metabolism in multiple shoot cultures of Ashwagandha (Withania somnifera Dunal)
Effect of cadmium stress on inductive enzymatic and nonenzymatic responses of ROS and sugar metabolism in multiple shoot cultures of Ashwagandha (Withania somnifera Dunal)
复制标题
DOI：
10.1007/s00709-014-0613-4
复制
发表时间：
2014-09-01
期刊：
影响因子：
2.9
通讯作者：
Sangwan, Neelam S.
中科院分区：
文献类型：
--
作者：
Mishra, Bhawana;Sangwan, Rajender S.;Sangwan, Neelam S.
Withania somnifera是最重要的药用植物之一，被认为具有多种药理活性。本研究以不同浓度（5-300 μ M）的镉（Cd）作为硫酸盐镉，在体外培养多茎，探讨其积累重金属离子的能力及其对代谢状态和适应性反应的影响。结果表明，添加Cd干扰了氮、磷和钾的吸收，造成高剂量Cd下的氮、磷和钾缺乏，并导致生长发育迟缓、黄化和坏死。研究表明，离体芽部Cd积累呈浓度依赖性。过氧化氢酶、抗坏血酸过氧化物酶、愈创木酚过氧化物酶、过氧化物酶、谷胱甘肽- s转移酶、谷胱甘肽过氧化物酶、单脱氢抗坏血酸还原酶和脱氢抗坏血酸还原酶的酶活性和同功酶模式在Cd暴露下发生了显著改变。Cd胁迫对糖代谢也有显著调节。光合色素、酚类物质、生育酚、黄酮类、还原性谷胱甘肽、非蛋白硫醇、抗坏血酸和脯氨酸等其他参数的含量均表现出主要的诱导反应，反映了它们的保护作用。结果表明，酶和非酶反应的相互作用构成了对Cd积累耐受的系统努力和对其胁迫影响的有效清除策略。
Withania somnifera is one of the most important medicinal plant and is credited with various pharmacological activities. In this study, in vitro multiple shoot cultures were exposed to different concentrations (5-300 mu M) of cadmium (Cd) as cadmium sulphate to explore its ability to accumulate the heavy metal ion and its impact on the metabolic status and adaptive responses. The results showed that supplemental exposure to Cd interfered with N, P, and K uptake creating N, P, and K deficiency at higher doses of Cd that also caused stunting of growth, chlorosis, and necrosis. The study showed that in vitro shoots could markedly accumulate Cd in a concentration-dependent manner. Enzymatic activities and isozymic pattern of catalase, ascorbate peroxidase, guaiacol peroxidase, peroxidase, glutathione-S-transferase, glutathione peroxidase, monodehydroascorbate reductase, and dehydroascorbate reductase were altered substantially under Cd exposure. Sugar metabolism was also markedly modulated under Cd stress. Various other parameters including contents of photosynthetic pigments, phenolics, tocopherol, flavonoids, reduced glutathione, nonprotein thiol, ascorbate, and proline displayed major inductive responses reflecting their protective role. The results showed that interplay of enzymatic as well as nonenzymatic responses constituted a system endeavor of tolerance of Cd accumulation and an efficient scavenging strategy of its stress implications.