Micro-CT and Histological Evaluation of an Neural Interface Implanted Within a Blood Vessel
Micro-CT and Histological Evaluation of an Neural Interface Implanted Within a Blood Vessel
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DOI：
10.1109/tbme.2016.2552226
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发表时间：
2017-04-01
影响因子：
4.6
通讯作者：
Oxley, Thomas J.
中科院分区：
文献类型：
--
作者：
Opie, Nicholas Lachlan;van der Nagel, Nicole R.;Oxley, Thomas J.
目的：最近，我们报道了一种支架安装电极阵列（Stentrode）的发展，该阵列能够长期高保真地记录血管内的神经信号。初步数据表明，将支架植入血管壁可提高记录灵敏度。我们现在研究支架的内膜结合，在一群绵羊中植入长达190天。方法：从澳大利亚同步加速器的成像和医学光束线获得的Micro-CT，与专门用于评估植入支架-电极阵列的脑血管系统的组织形态学技术进行比较，作为评估设备植入和血管通畅的措施。结果：显微ct分析和组织形态学分析显示，植入时间与植入支架的数量有很强的相关性。两种方式的12周具有可比性，显微ct测量339 +/- 15 μ m，组织学测量331 +/- 19 μ m （n = 292）。使用两种方式测量的管腔面积之间存在很强的相关性（p < 0.001），在植入长达190天的12只动物中没有观察到血管闭塞。结论：显微ct技术与组织形态测量技术具有可比性，均可用于血管支架植入的识别。意义：本研究初步证明了支架-电极阵列植入脑血管系统的安全性，可能促进微创脑机接口的技术进步。
Objective: Recently, we reported the development of a stent-mounted electrode array (Stentrode) capable of chronically recording neural signals from within a blood vessel with high fidelity. Preliminary data suggested incorporation of the Stentrode into the blood vessel wall was associated with improved recording sensitivity. We now investigate neointimal incorporation of the Stentrode, implanted in a cohort of sheep for up to 190 days. Methods: Micro-CT, obtained from the Imaging and Medical Beamline at the Australian Synchrotron, and histomorphometic techniques developed specifically for evaluation of cerebral vasculature implanted with a stent-electrode array were compared as measures to assess device incorporation and vessel patency. Results: Both micro-CT analysis and histomorphometry, revealed a strong correlation between implant duration and the number of incorporated stent struts. 12 weeks was comparable across both modalities, 339 +/- 15 mu m measured using micro-CT and 331 +/- 19 mu m (n = 292) measured histologically. There was a strong correlation between lumen areas measured using the two modalities (p < 0.001), with no observation of vessel occlusion observed from any of the 12 animals implanted for up to 190 days. Conclusion: Micro-CT and the histomorphometric techniques we developed are comparable and can both be used to identify incorporation of a Stentrode implanted in cerebral vessels. Significance: This study demonstrates preliminary safety of a stent-electrode array implanted in cerebral vasculature, which may facilitate technological advances in minimally invasive brain-computer interfaces.