ETTIN (ARF3) physically interacts with KANADI proteins to form a functional complex essential for integument development and polarity determination in Arabidopsis
ETTIN (ARF3) physically interacts with KANADI proteins to form a functional complex essential for integument development and polarity determination in Arabidopsis
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DOI：
10.1242/dev.067918
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发表时间：
2012-03-15
期刊：
影响因子：
4.6
通讯作者：
Gasser, Charles S.
中科院分区：
文献类型：
--
作者：
Kelley, Dior R.;Arreola, Alexandra;Gasser, Charles S.
KANADI（KAN）转录因子在整个植物发育过程中促进远轴细胞命运，并且在胚、叶、心皮和胚珠发育期间是器官形成所需的。在胚珠发育过程中，异常种皮形状（ATS，或KAN 4）是维持两个胚珠珠被之间的边界和促进内珠被生长所必需的。酵母双杂交试验确定ETTIN（ETT，或生长素反应因子3）作为一种转录因子，可以与ATS物理相互作用。ATS和ETT被证明是物理相互作用，在体内瞬时转化烟草表皮细胞使用双分子荧光互补。ATS和ETT被发现共享一个重叠的表达模式在拟南芥胚珠发育和损失的基因导致先天性融合珠被和改变种子形态。我们假设，在野生型胚珠的ATS和ETT之间的物理相互作用，使这些蛋白质采取一致行动，以确定珠被原基之间的边界。我们进一步显示了ETT和KAN 1（ATS的一种parastrophin）之间在酵母中的蛋白质-蛋白质相互作用。因此，ETT和KAN蛋白之间的直接物理关联支持了它们先前描述的在极性建立和器官发生中的共同作用。我们建议，ETT-KAN蛋白复合物（ES）构成的生长素依赖的调节模块，在各种发育环境中发挥保守的作用。
KANADI (KAN) transcription factors promote abaxial cell fate throughout plant development and are required for organ formation during embryo, leaf, carpel and ovule development. ABERRANT TESTA SHAPE (ATS, or KAN4) is necessary during ovule development to maintain the boundary between the two ovule integuments and to promote inner integument growth. Yeast two-hybrid assays identified ETTIN (ETT, or AUXIN RESPONSE FACTOR 3) as a transcription factor that could physically interact with ATS. ATS and ETT were shown to physically interact in vivo in transiently transformed tobacco epidermal cells using bimolecular fluorescence complementation. ATS and ETT were found to share an overlapping expression pattern during Arabidopsis ovule development and loss of either gene resulted in congenital fusion of the integuments and altered seed morphology. We hypothesize that in wild-type ovules a physical interaction between ATS and ETT allows these proteins to act in concert to define the boundary between integument primordia. We further show protein-protein interaction in yeast between ETT and KAN1, a paralog of ATS. Thus, a direct physical association between ETT and KAN proteins underpins their previously described common role in polarity establishment and organogenesis. We propose that ETT-KAN protein complex(es) constitute part of an auxin-dependent regulatory module that plays a conserved role in a variety of developmental contexts.