Single-cell RNA sequencing reveals the diversity and biology of valve cells in cardiac valve disease.
Single-cell RNA sequencing reveals the diversity and biology of valve cells in cardiac valve disease.
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DOI：
10.1016/j.jjcc.2022.03.012
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发表时间：
2022-04
影响因子：
2.5
通讯作者：
Mengxia Fu;Jia Song
中科院分区：
文献类型：
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作者：
Mengxia Fu;Jia Song
从高度排列的细胞外原纤维到细胞，瓣膜小叶的多层次有序结构对其生物学功能至关重要。心脏瓣膜病理最常见的是通过细胞、细胞外基质及其环境的异常和复杂的相互作用，破坏正常的结构功能相关性。目前，对瓣膜疾病的有效治疗是有限的，并且经常以手术修复或更换机械或人工生物心脏瓣膜而告终，这对于包括老年人和儿童在内的许多高危患者带来了不可克服的并发症。因此，迫切需要充分了解瓣膜疾病的病理生物学，以便开发更好的替代疗法。迄今为止，大多数研究都集中在细胞水平上描述瓣膜疾病的机制。然而，细胞的异质性和功能尚不清楚。在这篇综述中，我们总结了关于瓣膜细胞的工作，特别关注使用单细胞RNA测序对瓣膜细胞异质性和功能的发现。最后，我们讨论了破译这些复杂细胞类型异质性的最新策略，并认为这些知识可以转化为心脏瓣膜疾病的改进个性化治疗。
From highly aligned extracellular fibrils to the cells, a multilevel ordered hierarchy in valve leaflets is crucial for their biological function. Cardiac valve pathology most frequently involves a disruption in normal structure-function correlations through abnormal and complex interaction of cells, extracellular matrix, and their environment. At present, effective treatment for valve disease is limited and frequently ends with surgical repair or replacement with a mechanical or artificial biological cardiac valve, which comes with insuperable complications for many high-risk patients including aged and pediatric populations. Therefore, there is a critical need to fully appreciate the pathobiology of valve disease in order to develop better, alternative therapies. To date, the majority of studies have focused on delineating valve disease mechanisms at the cellular level. However, the cellular heterogeneity and function is still unclear. In this review, we summarize the body of work on valve cells, with a particular focus on the discoveries about valve cells heterogeneity and functions using single-cell RNA sequencing. We conclude by discussing state-of-the-art strategies for deciphering heterogeneity of these complex cell types, and argue this knowledge could translate into the improved personalized treatment of cardiac valve disease.