Interaction of inflammatory cells and oral microorganisms. IX. the bactericidal effects of human polymorphonuclear leukocytes on isolated plaque microorganisms.
Interaction of inflammatory cells and oral microorganisms. IX. the bactericidal effects of human polymorphonuclear leukocytes on isolated plaque microorganisms.
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炎症细胞和口腔微生物的相互作用。
DOI：
10.1111/j.1600-0765.1980.tb00305.x
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发表时间：
1980
影响因子：
3.5
通讯作者：
Taichman,NS
中科院分区：
文献类型：
--
作者：
Passo,SA;Tsai,CC;McArthur,WP;Leifer,C;Taichman,NS
多形核白细胞(PMN)与牙龈沟内的菌斑微生物接触。然而，对PMN杀灭或抑制斑块微生物生长的能力知之甚少。本研究对人中性粒细胞和血清的杀菌作用进行了研究。突变株6715，血链球菌M5，安达。对粘菌T14毒力和无毒株进行了检测。培养至固定相的菌斑微生物与人PMN和/或血清在37℃下孵育90分钟，通过测定菌落形成单位减少的百分比和对~3H-尿苷摄取的抑制百分比来确定对细菌的杀灭和生长抑制。在新鲜但未经热灭活(56℃，30分钟)的人血清存在下，人PMN对所有测试的微生物都有显著的杀菌作用。暴露OFS。变种6715，但不变。血M5对蔗糖在新鲜人血清存在下抑制人PMN的杀菌作用，但在兔抗变形免疫球蛋白存在下不抑制其杀菌作用。细胞松弛素B能明显抑制OFS的吞噬功能。人多形核细胞在血清存在下突变6715。然而，细胞松弛素B处理的中性粒细胞仍然具有显著的杀菌作用。在血清存在的情况下突变6715。这些数据表明，人的中性粒细胞可能会杀死或抑制牙周缝隙中某些菌斑微生物的生长。细菌胞外多糖以及血清抗体和补体的存在可能会影响微生物的杀灭作用，从而导致中性粒细胞的吞噬和胞外溶酶体释放。
Polymorphonuclear leukocytes (PMNs) come in contact with plaque microorganisms in the gingival sulcus. However, little is known about the ability of PMNs to kill or inhibit the growth of plaque microorganisms. In this study, the bactericidal effects of human PMNs and serum againstS. mutans6715,S. sanguisM5, andA. viscosusT14 virulent and avirulent were examined. Plaque microorganisms grown to stationary phase were incubated with human PMNs and/or serum for 90 minutes at 37°C. The killing and growth inhibition of bacteria were determined by measurement of percent reduction in colony forming units and percent inhibition of3H‐uridine uptake by the microorganisms.A. viscosusT14 virulent was the only microorganism significantly killed by human PMNs in the absence of serum. In the presence of fresh but not heat‐inactivated (56°C, 30 minutes) human serum, human PMNs had a significant bactericidal effect against all of the microorganisms tested. Exposure ofS. mutans6715, but notS. sanguisM5, to sucrose inhibited the bactericidal effect of human PMNs in the presence of fresh human serum but not in the presence of rabbit anti‐S. mutansimmunoglobulin. Cytochalasin B markedly inhibited phagocytosis ofS. mutans6715 by human PMNs in the presence of serum. However, cytochalasin B‐treated PMNs still were significantly bactericidal forS. mutans6715 in the presence of serum. The data suggests that human PMNs may kill or inhibit the growth of certain plaque microorganisms in the gingival crevice. The microbial killing may be influenced by bacterial extracellular polysaccharides and the presence of serum antibodies and complement, both resulting in phagocytosis and extracellular lysosomal release from PMNs.