Bacterial Proteases as Pathogenic Factors, with Special Emphasis on Vibrio Proteases
Bacterial Proteases as Pathogenic Factors, with Special Emphasis on Vibrio Proteases
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细菌蛋白酶作为致病因素，特别强调弧菌蛋白酶
DOI：
10.3109/15569549609064086
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
K. Tomochika
中科院分区：
文献类型：
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作者：
S. Shinoda;S. Miyoshi;H. Wakae;Monzur Rahman;K. Tomochika
摘要细菌蛋白酶在感染中发挥多种致病作用。弧菌属的致病菌，例如霍乱弧菌、副溶血弧菌或创伤弧菌也产生细胞外蛋白酶，并且它们几乎都是具有锌原子的金属蛋白酶。创伤弧菌是一种机会性人类病原体，可引起败血症或伤口感染，其特征是在皮肤上形成水肿、坏死性溃疡和蜂窝织炎。弧菌（VVP）产生的蛋白酶通过激活哈格曼因子-血浆激肽释放酶-激肽级联和/或肥大细胞胞吐组胺释放来增强血管通透性，并形成出血性病变，随后引起严重的皮肤坏死。因此，VVP 是最有可能在感染中形成水肿的候选者。还证明了VVP的其他致病作用。克隆了VVP基因，并从核苷酸序列推导出了氨基酸序列。成熟的蛋白质被证明由 413 个氨基酸残基组成......
AbstractBacterial proteases play various pathogenic roles in infection. Pathogenic species of the genus Vibrio, such as V. cholerae, V. parahaemolyticus or V. vulnificus also produce exocellular proteases, and almost of them are metalloproteases having a zinc atom. V. vulnificus is an opportunistic human pathogen causing septicemia or wound infections characterized by the formation of edema, necrotic ulcer and cellulitis on the skin. The protease produced by the vibrio (VVP) enhanced vascular permeability through activation of the Hageman factor-plasma kallikrein-kinin cascade and/or exocytotic histamine release from mast cells, and formed hemorrhagic lesions which subsequently provoke severe dermonecrosis. Thus, VVP is the most probable candidate for edema formation in the infection. Other pathogenic roles of VVP were also demonstrated.The VVP gene was cloned, and the amino acid sequence was deduced from the nucleotide sequence. The mature protein was demonstrated to be composed of 413 amino acid residue...