Bovine Nuclear Transfer Using Cumulus Cells Derived from Serum-Starved and Confluent Cultures
Bovine Nuclear Transfer Using Cumulus Cells Derived from Serum-Starved and Confluent Cultures
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使用源自血清饥饿和汇合培养物的卵丘细胞进行牛核移植
DOI：
10.1262/jrd.46.85
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发表时间：
2000
影响因子：
1.8
通讯作者：
Yoshiyuki Takahashi
中科院分区：
文献类型：
--
作者：
M. Nour;K. Ikeda;Yoshiyuki Takahashi
牛卵丘细胞成熟后，在血清饥饿条件下培养或生长至汇合。研究了它们的细胞大小、细胞周期时相和作为核移植（NT）供体的发育潜力。发现两种培养物的大多数胰蛋白酶消化细胞在中等大小范围内（15-20 μm）。流式细胞术分析显示，无论培养条件如何，小型和中型细胞具有显著更高的存在于G 0/G1期的细胞核百分比（95-98%），而大型细胞（82%）则无明显差异。不同大小（小、中、大）的血清饥饿细胞的融合率、卵裂率、囊胚发育率和囊胚细胞数均与NT胚胎相同。NT胚胎重建血清饥饿和融合的中等大小的细胞表现出相同的发育率。这些结果表明，大多数来自血清饥饿和汇合培养的中等大小的细胞可以用作G 0/G1期细胞核来源。
Bovine cumulus cells after oocyte maturation were cultured under serum-starvation condition or grown to confluence. Their cell size, cell cycle phases, and developmental potential as donors for nuclear transfer (NT) were investigated. The majority of trypsinized cells of both cultures were found within the medium size range (15-20 μm). Flow cytometric analysis revealed that small- and medium-sized cells had significantly higher percentages of nuclei existing in the G0/G1 phase (95-98%) than large-sized cells (82%) regardless of the culture conditions. Serum-starved cells of different sizes (small, 9-14 μm; medium, 15-20 μm; large, 21-26 μm) had the same fusion rate, cleavage, development to blastocysts, and blastocyst cell number of NT embryos. NT embryos reconstituted from serum-starved and confluent medium-sized cells showed the same developmental rate. These results indicate that the most available medium-sized cells derived from both serum-starvation and confluent cultures can be used as a G0/G1-phase nuclei source.
通过将培养的胎儿成纤维细胞显微注射到体外成熟卵母细胞中重建猪胚胎的发育。
DOI：
10.1016/s0378-4320(99)00037-8
发表时间：
1999
期刊：
Animal reproduction science.
影响因子：
--
作者：
Tao,T;Machaty,Z;Boquest,AC;Day,BN;Prather,RS
通讯作者：
Prather,RS
影响因子：
3.6
作者：
Boquest, AC;Day, BN;Prather, RS
通讯作者：
Prather, RS