19F Magnetic resonance imaging of perfluorooctanoic acid encapsulated in liposome for biodistribution measurement
19F Magnetic resonance imaging of perfluorooctanoic acid encapsulated in liposome for biodistribution measurement
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DOI：
10.1016/j.mri.2004.01.060
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发表时间：
2004-07-01
影响因子：
2.5
通讯作者：
Fujiwara, H
中科院分区：
文献类型：
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作者：
Kimura, A;Narazaki, M;Fujiwara, H
全氟辛酸（PFOA），这是已知的，显示独特的生物反应，已可视化的组织分布在雌性小鼠的F-19磁共振成像（MRI）结合显微成像技术的最新进展。应用化学位移选择性快速自旋回波法获得活体PFOA的F-19 MR图像。PFOA的体内T-1和T-2弛豫时间被证明非常短，分别为140（+/- 20）ms和6.3（+/- 2.2）ms。为了获得活体F-19 PFOA的MR图像，需要优化信号选择和回波链长度的参数。利用PFOA的CF 3基团的F-19 NMR信号进行有效的化学位移选择，而没有信号重叠，因为CF 3和相邻信号之间的化学位移差达到14 kHz。确定有效获得F-19图像的最佳回波链长度，以便快速自旋回波序列中的最大回波时间（TE）值与体内T-2值相当。通过优化这些参数，能够在12分钟内有效地获得PFOA的体内F-19 MR图像。因此，在F-19 MR图像中清楚地追踪了PFOA在小鼠肝脏中积累的时间过程。因此，可以得出结论，F-19 MRI成为未来全氟羧酸的药理学和毒理学研究的有效方法。(C)2004年爱思唯尔公司All rights reserved.
The tissue distribution of perfluorooctanoic acid (PFOA), which is known to show unique biological responses, has been visualized in female mice by F-19 magnetic resonance imaging (MRI) incorporated with the recent advances in microimaging technique. The chemical shift selected fast spin-echo method was applied to acquire in vivo F-19 MR images of PFOA. The in vivo T-1 and T-2 relaxation times of PFOA were proven to be extremely short, which were 140 (+/- 20) ms and 6.3 (+/- 2.2) ms, respectively. To acquire the in vivo F-19 MR images of PFOA, it was necessary to optimize the parameters of signal selection and echo train length. The chemical shift selection was effectively performed by using the F-19 NMR signal of CF3 group of PFOA without the signal overlapping because the chemical shift difference between the CF3 and neighbor signals reaches to 14kHz. The most optimal echo train length to obtain F-19 images efficiently was determined so that the maximum echo time (TE) value in the fast spin-echo sequence was comparable to the in vivo T-2 value. By optimizing these parameters, the in vivo F-19 MR image of PFOA was enabled to obtain efficiently in 12 minutes. As a result, the time course of the accumulation of PFOA into the mouse liver was clearly pursued in the F-19 MR images. Thus, it was concluded that the F-19 MRI becomes the effective method toward the future pharmacological and toxicological studies of perfluorocarboxilic acids. (C) 2004 Elsevier Inc. All rights reserved.