Comparative analysis of gene expression among low G+C gram-positive genomes
Comparative analysis of gene expression among low G+C gram-positive genomes
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DOI：
10.1073/pnas.0401504101
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发表时间：
2004-04-20
影响因子：
11.1
通讯作者：
Mrázek, J
中科院分区：
文献类型：
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作者：
Karlin, S;Theriot, J;Mrázek, J
我们提出了一个预测的高表达（PHX）基因在低G+C革兰氏阳性基因组的枯草芽孢杆菌，耐盐芽孢杆菌，单核细胞增生李斯特菌，无害李斯特菌，乳酸乳球菌，酿脓链球菌，肺炎链球菌，金黄色葡萄球菌，梭菌，丙酮丁醇，产气荚膜梭菌的比较分析。大多数在糖酵解和发酵途径中起作用的酶在这些基因组中是PHX，但不是那些参与TCA循环和呼吸的酶，这表明这些生物主要适应在厌氧环境中快速生长。只有B。枯草芽孢杆菌和B.嗜盐链球菌具有几个TCA循环PHX基因，而TCA途径在两种链球菌属物种的代谢库中完全缺失，并且在李斯特菌属、乳球菌属和梭菌属中不完整。丙酮酸-甲酸裂解酶是混合酸发酵中的关键酶，是除C.丙酮丁醇（非PHX），和B。subtilis和B. B. halodurans（缺失）。丙酮酸-甲酸裂解酶在肠道γ-变形菌中也是显著的PHX，但在其他原核生物中不是。磷酸转移酶系统基因通常是PHX，在不同的基因组中选择不同的底物。不同基因组中磷酸转移酶系统之间的各种底物特异性显然反映了栖息地，生活方式和营养来源的差异。
We present a comparative analysis of predicted highly expressed (PHX) genes in the low G+C Gram-positive genomes of Bacillus subtilis, Bacillus halodurans, Listeria monocytogenes, Listeria innocua, Lactococcus lactis, Streptococcus pyogenes, Streptococcus pneumoniae, Staphylococcus aureus, Clostridium, acetobutylicum, and Clostridium perfringens. Most enzymes acting in glycolysis and fermentation pathways are PHX in these genomes, but not those involved in the TCA cycle and respiration, suggesting that these organisms have predominantly adapted to grow rapidly in an anaerobic environment. Only B. subtilis and B. halodurans have several TCA cycle PHX genes, whereas the TCA pathway is entirely missing from the metabolic repertoire of the two Streptococcus species and is incomplete in Listeria, Lactococcus, and Clostridium. Pyruvate-formate lyase, an enzyme critical in mixed acid fermentation, is among the highest PHX genes in all these genomes except for C. acetobutylicum (not PHX), and B. subtilis, and B. halodurans (missing). Pyruvate-formate lyase is also prominently PHX in enteric gamma-proteobacteria, but not in other prokaryotes. Phosphotransferase system genes are generally PHX with selection of different substrates in different genomes. The various substrate specificities among phosphotransferase systems in different genomes apparently reflect on differences in habitat, lifestyle, and nutrient sources.