Detection of 881A→881G mutation in tyrosinase gene and associations with the black ear coat color in rabbits
Detection of 881A→881G mutation in tyrosinase gene and associations with the black ear coat color in rabbits
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DOI：
10.5713/ajas.2002.1395
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发表时间：
2002-10-01
期刊：
影响因子：
--
通讯作者：
Du, LX
中科院分区：
文献类型：
--
作者：
Jiang, YL;Fan, XZ;Du, LX
选择酪氨酸酶基因作为揭示家兔“黑耳”毛色遗传机制的候选基因。建立了位于酪氨酸酶基因中心区域CuA和CuB结合区特征之间的881(A)—>881(G)突变的PCR-SSCP检测方法，并通过测序和比对得到证实。通过对“黑耳”谱系和61个无关个体的分析，观察到SNP与“黑耳”毛色之间完全一致的关联。该SNP可作为“后耳”毛兔育种的分子标记。
The tyrosinase gene was selected as a candidate for uncovering genetic mechanism causing "black ear" coat color in rabbits. A PCR-SSCP detection method was established for the 881(A)-->881(G) mutation located in the central region of the tyrosinase gene between the CuA and CuB binding region signatures, and this was confirmed by sequencing and alignment. Fully consistent associations between the SNP and "black ear" coat color were observed by analysis in a "black ear" pedigree and on 61 unrelated individuals. This SNP can serve as a molecular marker for Use in "back ear" wool rabbit breeding.