MOLECULAR-STRUCTURE OF THE HUMAN DESMOPLAKIN-I AND DESMOPLAKIN-II AMINO TERMINUS
MOLECULAR-STRUCTURE OF THE HUMAN DESMOPLAKIN-I AND DESMOPLAKIN-II AMINO TERMINUS
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DOI：
10.1073/pnas.89.2.544
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发表时间：
1992-01-15
影响因子：
11.1
通讯作者：
GREEN, KJ
中科院分区：
文献类型：
--
作者：
VIRATA, MLA;WAGNER, RM;GREEN, KJ
桥粒蛋白（Desmoplakins, DPs） I和II是在桥粒斑块最内层区域发现的密切相关的蛋白，是细胞质中间丝的细胞表面附着位点。重叠的cDNA克隆包含9.2千碱基的DP-I，预测编码全长310 kda的多肽（2677个氨基酸残基），现在已经确定。在这里，我们报道了预测的蛋白质序列和DP的N端结构分析，扩展了我们之前对棒和羧基结构域的研究。N端包含七核苷酸重复组，预计至少形成两个主要的富含α -螺旋的束。与棒状结构域和羧基结构域不同，N端没有显示带电残基的周期性分布。还进行了Northern blot定位和基因组序列分析，以检查DP mrna的组织。一个1千碱基的内含子位于dp - 1特异性区域的3′边界；然而，在一个潜在的编码序列中观察到一个可能的剪接供体位点，而不是在5'连接处的内含子，这表明另一个RNA剪接来自内部的供体位点。
Desmoplakins (DPs) I and II are closely related proteins found in the innermost region of the desmosomal plaque, which serves as a cell surface attachment site for cytoplasmic intermediate filaments. Overlapping cDNA clones comprising 9.2 kilobases of DP-I, predicted to encode a full-length 310-kDa polypeptide (2677 amino acid residues), have now been identified. Here we report the predicted protein sequence and structural analysis of the N terminus of DP, extending our previous study of the rod and carboxyl domains. The N terminus contains groups of heptad repeats that are predicted to form at least two major alpha-helical-rich bundles. Unlike the rod and carboxyl domains, the N terminus did not display a periodic distribution of charged residues. Northern blot mapping and genomic sequence analysis were also undertaken to examine the organization of the DP mRNAs. A 1-kilobase intron was located at the 3' boundary of a DP-I-specific region; however, instead of an intron at the 5' junction, a possible splice donor site was observed within a potential coding sequence, suggesting alternative RNA splicing from an internal donor site.