EU-OSTID:: A collection of transposon insertional mutants for functional genomics in rice
EU-OSTID:: A collection of transposon insertional mutants for functional genomics in rice
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DOI：
10.1007/s11103-005-8532-6
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发表时间：
2005-09-01
影响因子：
5.1
通讯作者：
Pereira, A
中科院分区：
文献类型：
--
作者：
van Enckevort, LJG;Droc, G;Pereira, A
从Ac/Ds转座子系和Ac转座子系中分别获得了1373个用于反向遗传学研究的独特侧翼序列标签（FSTs）。Ds和Ac FST与原始T-DNA一起被分配到水稻基因组序列中的一个位置，表示为组装的假分子，并发现均匀分布在整个水稻基因组中，对预测的基因丰富区域具有明显的偏好。Ds和Ac转座子插入基因的偏差的例证是存在的插入59%的基因注释的水稻染色体上和41%的基因转录的同源性公开的cDNA克隆。在一组75个注释良好的转录因子，包括同源框含有基因的插入筛选中，我们发现了6个Ac/Ds插入。基因中Ds和Ac插入的这种高频率表明，使用这种策略在水稻中进行饱和敲除诱变将是有效的，并且可能具有比预期更低的插入数。这些FST和相应的植物品系可通过OrygenesDB数据库和欧盟联盟成员公开获得。
A collection of 1373 unique flanking sequence tags (FSTs), generated from Ac/Ds and Ac transposon lines for reverse genetics studies, were produced in japonica and indica rice, respectively. The Ds and Ac FSTs together with the original T-DNAs were assigned a position in the rice genome sequence represented as assembled pseudomolecules, and found to be distributed evenly over the entire rice genome with a distinct bias for predicted gene-rich regions. The bias of the Ds and Ac transposon inserts for genes was exemplified by the presence of 59% of the inserts in genes annotated on the rice chromosomes and 41% present in genes transcribed as disclosed by their homology to cDNA clones. In a screen for inserts in a set of 75 well annotated transcription factors, including homeobox-containing genes, we found six Ac/Ds inserts. This high frequency of Ds and Ac inserts in genes suggests that saturated knockout mutagenesis in rice using this strategy will be efficient and possible with a lower number of inserts than expected. These FSTs and the corresponding plant lines are publicly available through OrygenesDB database and from the EU consortium members.