Chemotaxis of human tonsil B lymphocytes to CC chemokine receptor (CCR) 1, CCR2 and CCR4 ligands is restricted to non-germinal center cells
Chemotaxis of human tonsil B lymphocytes to CC chemokine receptor (CCR) 1, CCR2 and CCR4 ligands is restricted to non-germinal center cells
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DOI：
10.1093/intimm/dxf054
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发表时间：
2002-08-01
影响因子：
4.4
通讯作者：
Pistoia, V
中科院分区：
文献类型：
--
作者：
Corcione, A;Tortolina, G;Pistoia, V
我们研究了9种CC趋化因子，即巨噬细胞炎性蛋白（MIP）-1 α/CCL 3、MIP-1 β/CCL 4、MIP-3 α/CCL 20、MIP-5/CCL 15、单核细胞趋化蛋白（MCP）-1/CCL 2、MCP-2/CCL 8、MCP-3/CCL 7、嗜酸性粒细胞趋化因子（eotaxin）/CCL 11和巨噬细胞源性趋化因子（MDC）/CCL 22对人扁桃体B淋巴细胞及其亚群运动的影响。在分离时，B细胞反应性差，但在短期培养后，它们对MIP-1 α、MIP-5、MCP-1、MCP-2、MCP-3和MDC显示出统计学显著的趋化反应（P < 0.001）。CC趋化因子受体（CCR）1至CCR 6在培养后上调。MIP-1 β、MIP-3 α和嗜酸性粒细胞趋化因子不刺激B细胞迁移。关于B细胞亚群对趋化因子的反应的零散信息是可用的。因此，我们研究了MIP-1 α、MIP-5、MCP-1、MCP-2、MCP-3和MDC对非生发中心（GC）（CD 38（-））和GC（CD 38（+））B细胞体外运动的影响。所有趋化因子均能显著增强前者的迁移（P < 0.001），但对后者无显著影响。通过流式细胞术在非GC（即幼稚和记忆）B细胞上检测到CCR 1、CCR 2和CCR 4，而它们在GC B细胞上不存在（CCR 1和CCR 2）或表达差（CCR 4）。
We have investigated the effects of nine CC chemokines, i.e. macrophage inflammatory protein (MIP)-1alpha/CCL3, MIP-1beta/CCL4, MIP-3alpha/CCL20, MIP-5/CCL15, monocyte chemotactic protein (MCP)-1/CCL2, MCP-2/CCL8, MCP-3/CCL7, eotaxin/CCL11 and macrophage-derived chemokine (MDC)/CCL22 on the locomotion of human tonsil B lymphocytes and their subsets. Upon isolation, B cells were poorly responsive, but, following short-term culture, they displayed statistically significant chemotactic responses (P < 0.001) to MIP-1alpha, MIP-5, MCP-1, MCP-2, MCP-3 and MDC. CC chemokine receptor (CCR) 1 to CCR6 were up-regulated after culture. MIP-1beta, MIP-3alpha and eotaxin did not stimulate B cell migration. Scattered information is available on B cell subset responses to chemokines. Therefore, we investigated the effects of MIP-1alpha, MIP-5, MCP-1, MCP-2, MCP-3 and MDC on the in vitro locomotion of non-germinal center (GC) (CD38(-)) and GC (CD38(+)) B cells. All chemokines enhanced significantly (P < 0.001) the migration of the former, but not of the latter, cells. CCR1, CCR2 and CCR4 were detected by flow cytometry on non-GC (i.e. naive and memory) B cells, whereas they were absent (CCR1 and CCR2) or poorly expressed (CCR4) on GC B cells.