BIOANALYTICAL APPLICATIONS OF FLUORESCENCE SPECTROSCOPY
BIOANALYTICAL APPLICATIONS OF FLUORESCENCE SPECTROSCOPY
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DOI：
10.1021/ac00169a001
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发表时间：
1988-09-15
影响因子：
7.4
通讯作者：
BRIGHT, FV
中科院分区：
文献类型：
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作者：
BRIGHT, FV
荧光光谱是一种非常强大的分析工具，已被用于从生物学到物理学的各个领域。在这份报告中，我们将介绍荧光光谱在生物分析系统中的应用，包括DNA和RNA测定、酶法、蛋白质-配体相互作用、荧光免疫分析和光纤传感。由于篇幅所限，无法提及其他相关领域（例如，液相色谱中的荧光检测）。本文分为以下几个部分：历史回顾、理论、仪器、优点、应用和未来。
Fluorescence spectroscopy is an ex-tremely powerful analytical tool that has been used in areas ranging from biology to physics. In this REPORT, we will present applications of fluores-cence spectroscopy to bioanalytical systems, including DNA and RNA de-terminations, enzymatic methods, pro-tein-ligand interactions, fluoroimmun-oassays, and fiber-optic-based sensing. Space limitations preclude mention of other relevant areas(eg, fluorescence detection in liquid chromatography). The article is divided into the following sections: historical perspective, theory, instrumentation, advantages of fluo-rescence, applications, and the future.