Vascular endothelial growth factor (VEGF) receptor neuropilin-1's distribution in astrocytic tumors
Vascular endothelial growth factor (VEGF) receptor neuropilin-1's distribution in astrocytic tumors
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DOI：
10.1111/j.1600-0463.2004.apm11204-0505.x
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发表时间：
2004-04-01
期刊：
影响因子：
2.8
通讯作者：
Laursen, H
中科院分区：
文献类型：
--
作者：
Broholm, H;Laursen, H
Neuropilin-1是由内皮细胞和肿瘤细胞表达的VEGF（165）和脑信号蛋白受体。神经纤毛蛋白-1的具体功能尚不完全清楚，但在发育中的神经系统中，神经纤毛蛋白作为一种脑信号蛋白受体，已被证明影响神经元的引导。神经纤毛蛋白-1的表达在低级别和高级别星形细胞肿瘤中进行了研究，后者的特点是广泛的血管生成。我们对20例低级别星形细胞瘤（WHO II级）和46例胶质母细胞瘤（WHO IV级）进行了神经纤毛蛋白-1，p53和EGFR的化学检测。胶质母细胞瘤根据p53和EGFR的表达分为原发性胶质母细胞瘤35例，继发性胶质母细胞瘤9例和2例不确定病例。此外，评估肥大细胞的存在以寻找血管生成中的任何潜在功能。胶质母细胞瘤在增殖血管的内皮细胞中表达neuropilin-1，并且大多数胶质母细胞瘤具有免疫反应性肿瘤性星形胶质细胞，胶质母细胞瘤亚组之间没有差异。20例低级别星形细胞瘤中有6例内皮细胞呈阴性，20例肿瘤细胞中有8例神经纤毛蛋白-1呈阴性。肥大细胞在软脑膜较大血管周围的胶原基质中观察到，但不邻近恶性肿瘤血管或作为肿瘤过程本身的一部分。神经纤毛蛋白-1的表达增加显示在胶质母细胞瘤的内皮细胞和肿瘤性星形胶质细胞中。在肿瘤性星形胶质细胞和内皮细胞中，大约一半的低级别星形细胞瘤中神经纤毛蛋白-1表达较少。结果表明，神经纤毛蛋白-1和血管在人类星形细胞肿瘤和神经纤毛蛋白-1作为VEGF诱导的血管生成的受体可能的作用之间的相关性。
Neuropilin-1 is a VEGF(165)- and semaphorin receptor expressed by endothelial cells and tumor cells. The specific function of neuropilin-1 is not fully known, but in the developing nervous system neuropilin, as a semaphorin receptor, has been shown to influence neuronal guidance. The expression of neuropilin-1 was studied in low-grade and high-grade astrocytic tumors, the latter characterized by extensive angiogenesis. We examined 20 low-grade astrocytomas (WHO grade II) and 46 glioblastomas (WHO grade IV) immunohistochemically for neuropilin-1, p53 and EGFR. The glioblastomas were according to the p53 and EGFR expression classified as 35 primary - de novo - glioblastomas, 9 secondary glioblastomas, and 2 uncertain cases. Furthermore, the presence of mast cells was evaluated to search for any potential function in angiogenesis. The glioblastomas expressed neuropilin-1 in the endothelial cells of the proliferating vessels and the majority of the glioblastomas had immunoreactive neoplastic astrocytes, with no difference between the glioblastoma subgroups. Six out of twenty of the low-grade astrocytomas were negative in the endothelial cells and 8 out of 20 in the tumor cells for neuropilin-1. Mast cells were observed in the collagen matrix around larger vessels in the leptomeninges, but not adjacent to malignant tumor vessels or as part of the tumor process itself. Increased expression of neuropilin-1 is shown in endothelial cells and in neoplastic astrocytes of glioblastomas. Less neuropilin-1 expression is found in about half of the low-grade astrocytomas in both neoplastic astrocytes and endothelial cells. The results suggest a correlation between neuropilin-1 and vascularity in human astrocytic tumors and a possible role for neuropilin-1 as a receptor for VEGF-induced angiogenesis.