Quantitative orientation-independent differential interference contrast microscope with fast switching shear direction and bias modulation.
Quantitative orientation-independent differential interference contrast microscope with fast switching shear direction and bias modulation.
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DOI：
10.1364/josaa.30.000769
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发表时间：
2013-04-01
期刊：
影响因子：
--
通讯作者：
Shribak M
中科院分区：
文献类型：
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作者：
Shribak M
我们描述了定量方向无关的微分干涉相衬（OI-DIC）显微镜，它允许调制偏置延迟并快速切换剪切方向，而无需任何机械移动。剪切方向由夹在两个标准 DIC 棱镜之间的常规液晶盒切换。另一个液晶单元调节偏压。显示了测量 DIC 棱镜参数和校准偏差的技术。一秒钟内捕获两组具有正交剪切方向的原始 DIC 图像。然后计算薄光学截面内光路梯度分布的定量图像。梯度数据用于获得光路的定量分布，表示折射率梯度或高度分布。计算具有任何所需剪切方向的增强的常规 DIC 图像也是可能的。
We describe quantitative orientation-independent differential interference contrast (OI-DIC) microscope, which allows the bias retardation to be modulated and shear directions to be switched rapidly without any mechanically moving. The shear direction is switched by regular liquid crystal cell sandwiched between two standard DIC prisms. Another liquid crystal cell modulates the bias. Techniques for measuring parameters of DIC prisms and calibrating the bias are shown. Two sets of raw DIC images with the orthogonal shear directions are captured within a second. Then the quantitative image of optical path gradient distribution within a thin optical section is computed. The gradient data are used to obtain quantitative distribution of optical path, which represents refractive index gradient or height distribution. Computing enhanced regular DIC images with any desired shear direction is also possible.