The ubiquitin pathway for protein degradation.
The ubiquitin pathway for protein degradation.
复制标题
蛋白质降解的泛素途径。
DOI：
10.1016/0968-0004(91)90101-z
复制
发表时间：
1991
影响因子：
13.8
通讯作者：
Hershko,A
中科院分区：
文献类型：
--
作者：
Hershko,A
INTRACF。1 . LULAR蛋白的分解对细胞功能很重要，如调节调节蛋白的水平和去除异常蛋白。这一过程的生化机制长期以来一直不清楚。早期的观察表明，细胞蛋白质的降解需要代谢能量。对网状细胞atp依赖性蛋白水解系统作用方式的研究发现了选择性蛋白降解的主要途径。拆分和重构实验表明，该体系的作用需要一种热稳定的多肽。该多肽随后被鉴定为泛素，由76个氨基酸组成，在进化中具有高度保守性。然后发现多肽在需要atp的过程中与蛋白质底物共价连接。有人提出，蛋白质通过与泛素的连接而致力于降解。泛素系统的发现在参考文献1中进行了回顾，随后的生化工作在参考文献2中进行了回顾。本文综述了近年来有关泛素-蛋白连接选择性机制和泛素结合蛋白降解模式的研究进展。
INTRACF. I. LULAR PROTEIN breakdown is important for cellular functions such as the modulation of the levels of regulatory proteins and the removal of abnormal proteins. The biochemical mechanisms involved in this process were for a long time unclear. Early observations suggested that metabolic energy was required for the degradation of cellular proteins. Studies on the mode of action of an ATP-dependent proteolytic system from reticulocytes led to the discovery of a major pathway for selective protein degradation. Resolution and reconstitution experiments showed that a heat-stable polypeptide was required for the action of this system. This polypeptide was subsequently identified as ubiquitin, which consisted of 76 amino acids and was highly conserved in evolution. It was then found that the polypeptide is covalently linked to protein substrates in an ATP-requiring process. It was proposed that proteins are committed for degradation by their ligation to ubiquitin. The discovery of the ubiquitin system was reviewed in Ref. I and subsequent biochemical work has been reviewed in Ref. 2. In this review, recent information on the mechanisms of the selectivity of ubiquitin-protein ligation and on the mode of degradation of proteins conjugated to ubiquitin is discussed.
登录
查看更多内容
泛素-蛋白质连接酶系统的蛋白质底物结合位点。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hershko,A;Heller,H;Eytan,E;Reiss,Y
通讯作者：
Reiss,Y
DOI：
--
发表时间：
1983-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Hershko;H. Heller;S. Elias;A. Ciechanover
通讯作者：
A. Hershko;H. Heller;S. Elias;A. Ciechanover
降解与泛素连接的蛋白质的 26 S 复合物的组装伴随着 ATP 酶活性的形成。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Armon,T;Ganoth,D;Hershko,A
通讯作者：
Hershko,A
DOI：
10.1016/s0021-9258(18)37771-8
发表时间：
1988-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
D. Ganoth;Esther Leshinsky;E. Eytan;A. Hershko
通讯作者：
D. Ganoth;Esther Leshinsky;E. Eytan;A. Hershko
DOI：
10.1016/0006-291x(85)91050-2
发表时间：
1985-01-01
影响因子：
3.1
作者：
HERSHKO, A;HELLER, H
通讯作者：
HELLER, H