Role of Exopolymeric Substances (EPS) in the Stability of the Biofilm of Thiomonas arsenivorans Grown on a Porous Mineral Support
Role of Exopolymeric Substances (EPS) in the Stability of the Biofilm of Thiomonas arsenivorans Grown on a Porous Mineral Support
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DOI：
10.4014/jmb.1001.01017
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发表时间：
2011-02-01
影响因子：
2.8
通讯作者：
Dictor, Marie-Christine
中科院分区：
文献类型：
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作者：
Michel, Caroline;Garrido, Francis;Dictor, Marie-Christine
选择生物化学方法来评估外聚物在生物修复过程中使用的生物膜稳定性中的作用。因此，用链霉蛋白酶（蛋白质靶标）、凝集素（Con A或PNA）、荧光钙或高碘酸（多糖靶标）、DNA酶（DNA靶标）和脂肪酶（甘油三酯靶标）处理在火山灰上形成的食砷硫单胞菌的生物膜。蛋白酶和DNA酶处理对细菌粘附都没有任何影响。凝集素和calcofluor治疗主要影响年轻的生物膜。脂肪酶处理对生物膜的稳定性有显着的影响，无论生物膜的年龄。结果表明，这将是一个成熟的生物膜，保护他们免受外部攻击的阻力增加。
Biochemical methods were selected to evaluate the role of exopolymeric substances in the stability of biofilms used in bioremediation processes. Biofilms of Thiomonas arsenivorans formed on pozzolana were thus treated with pronase (protein target), lectins (Con A or PNA), calcofluor or periodic acid (polysaccharides target), DNase (DNA target), and lipase (triglycerides target). Neither protease nor DNase treatments had any effect on bacterial adhesion. Lectins and calcofluor treatments mainly affected young biofilms. Lipase treatment had a noticeable effect on biofilm stability whatever the biofilm age. Results suggest that it would be an increased resistance of mature biofilms that protects them from external attacks.