Real-time detection of mesothelin in pancreatic cancer cell line supernatant using an acoustic wave immunosensor
Real-time detection of mesothelin in pancreatic cancer cell line supernatant using an acoustic wave immunosensor
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DOI：
10.1016/j.cdp.2006.03.004
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发表时间：
2006-01-01
影响因子：
--
通讯作者：
Hunt, William D.
中科院分区：
文献类型：
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作者：
Corso, Christopher D.;Stubbs, Desmond D.;Hunt, William D.
Background: An acoustic wave immunosensor was developed to illustrate the viability of such devices in early detection of molecular cancer biomarkers. The methods described here involve a real-time, less invasive technique for detecting mesothelin, a protein that has been linked to pancreatic and ovarian cancer. Methods: Antibodies were immobilized on the gold surface of the device via a self-assembled alkanethiol monolayer. Supernatant from two different pancreatic cancer cell-lines (PL1 and CAPAN2) containing an unknown concentration of mesothelin was tested for the protein by a flow-through analytical technique in three types of experiments. Binding of the mesothelin to the immobilized antibody layer caused a shift in the device's resonant frequency, which was correlated to the concentration of supernatant. A reference sensor was used to correct for frequency shifts caused by pressure or viscosity effects from the injection of the supernatant solution. Results: Repeated experiments indicate that the sensors are capable of nanogram detection thresholds of mesothelin proteins at room temperature and in complex mixture. Conclusions: Acoustic wave device biosensors have the potential to become a valuable tool in screening for pancreatic as well as other types of cancers. The main features include real-time detection, high sensitivity, and ease of use. (c) 2006 International Society for Preventive Oncology. Published by Elsevier Ltd. All rights reserved.
