MIGRATION ASSAY FOR ENDOTHELIAL-CELLS IN MULTIWELLS - APPLICATION TO STUDIES ON THE EFFECT OF OPIOIDS
MIGRATION ASSAY FOR ENDOTHELIAL-CELLS IN MULTIWELLS - APPLICATION TO STUDIES ON THE EFFECT OF OPIOIDS
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DOI：
10.1016/0022-1759(90)90215-h
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发表时间：
1990-04-17
影响因子：
2.2
通讯作者：
KIRKPATRICK, CJ
中科院分区：
文献类型：
--
作者：
FISCHER, EG;STINGL, A;KIRKPATRICK, CJ
描述了一种测定系统，该系统允许使用单层生长的细胞快速有效地评估内皮细胞修复。通过这种方法，可以获得非常显着的结果。例如，内皮生长因子和肝素显着增强细胞迁移和/或增殖，而β-内啡肽(一种内源性阿片样物质)对人脐静脉内皮细胞的迁移和/或增殖没有影响。该模型可用于研究在某些实验条件（例如辐射）下不增殖的多种细胞类型的细胞迁移。
An assay system is described which permits rapid and effective evaluation of endothelial cell repair, using cells growing in a monolayer. With this method it was possible to obtain highly significant results. For example, endothelial growth factor and heparin, significantly enhanced cell migration and/or proliferation, whereas .beta.-endorphin, an endogenous opioid, had no effect on the migration and/or proliferation of human umbilical vein endothelial cells. This model may be used to study the cell migration of a variety of cell types which under certain experimental conditions (e.g., irradiation) do not proliferate.