Quantification of the Antioxidant Activity of Plant Extracts: Analysis of Sensitivity and Hierarchization Based on the Method Used
Quantification of the Antioxidant Activity of Plant Extracts: Analysis of Sensitivity and Hierarchization Based on the Method Used
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DOI：
10.3390/antiox9010076
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发表时间：
2020-01-01
期刊：
影响因子：
7
通讯作者：
Carlos Alias, Juan
中科院分区：
文献类型：
--
作者：
Chaves, Natividad;Santiago, Antonio;Carlos Alias, Juan
植物中含有大量具有较高抗氧化活性的生物活性化合物。研究不同植物的抗氧化活性有助于揭示这些植物作为新的抗氧化化合物来源的价值。体外测定抗氧化活性的方法多种多样，重要的是要选择合适的方法来确定哪些物种具有最高的抗氧化活性。本工作的目的是验证不同的方法是否具有相同的敏感性和/或能力来区分不同植物提取物的抗氧化活性。为此，我们选择了12种不同含量的酚类化合物。采用2,2-二苯基-1-苦基肼基（DPPH）自由基清除能力法、铁还原（FRAP）法、Trolox等效抗氧化能力（ABTS）法和还原力（RP）法对其提取物进行分析。所选择的4种方法虽然存在差异，但均可量化12种研究物种的抗氧化能力。DPPH和RP法测定的抗氧化活性值高于ABTS和FRAP法测定的抗氧化活性值，且不同种属之间存在差异。因此，这些物种的等级或分类因使用的方法而异。这些方法之间的另一个差异是每种方法获得的灵敏度。聚类结果表明，RP在离根最短距离处建立的群数最多。因此，由于RP对物种间差异和/或相似性的辨别效果最好，本研究认为RP是四种研究方法中灵敏度最高的方法。另一方面，ABTS的灵敏度最低。这些结果表明，选择合适的抗氧化活性定量方法对于建立基于该参数的物种排序的重要性。
Plants have a large number of bioactive compounds with high antioxidant activity. Studies for the determination of the antioxidant activity of different plant species could contribute to revealing the value of these species as a source of new antioxidant compounds. There is a large variety of in vitro methods to quantify antioxidant activity, and it is important to select the proper method to determine which species have the highest antioxidant activity. The aim of this work was to verify whether different methods show the same sensitivity and/or capacity to discriminate the antioxidant activity of the extract of different plant species. To that end, we selected 12 species with different content of phenolic compounds. Their extracts were analyzed using the following methods: 2,2-di-phenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay, ferric reducing (FRAP) assay, Trolox equivalent antioxidant capacity (ABTS) assay, and reducing power (RP) assay. The four methods selected could quantify the antioxidant capacity of the 12 study species, although there were differences between them. The antioxidant activity values quantified through DPPH and RP were higher than the ones obtained by ABTS and FRAP, and these values varied among species. Thus, the hierarchization or categorization of these species was different depending on the method used. Another difference established between these methods was the sensitivity obtained with each of them. A cluster revealed that RP established the largest number of groups at the shortest distance from the root. Therefore, as it showed the best discrimination of differences and/or similarities between species, RP is considered in this study as the one with the highest sensitivity among the four studied methods. On the other hand, ABTS showed the lowest sensitivity. These results show the importance of selecting the proper antioxidant activity quantification method for establishing a ranking of species based on this parameter.