HaloTag protein-mediated specific labeling of living cells with quantum dots
HaloTag protein-mediated specific labeling of living cells with quantum dots
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DOI：
10.1016/j.bbrc.2008.07.004
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发表时间：
2008-09-26
影响因子：
3.1
通讯作者：
Rao, Jianghong
中科院分区：
文献类型：
--
作者：
So, Min-Kyung;Yao, Hequan;Rao, Jianghong
量子点作为小分子荧光团的有吸引力的替代物出现，作为用于体内细胞标记和成像的荧光标记物。该通信提出了一种使用量子点特异性标记活细胞的方法。标记由在细胞表面表达的HaloTag蛋白介导，其与其配体（HaloTag配体）形成稳定的共价加合物。标记可以用用HaloTag配体官能化的量子点缀合物在一个单一步骤中进行，或者在两个步骤中首先用生物素化的HaloTag配体，然后用链霉亲和素包被的量子点进行。活细胞荧光成像表明标记是特异性的，并且发生在细胞表面。这种HaloTag蛋白介导的细胞标记方法应有助于量子点在活细胞成像中的应用。(C)2008年爱思唯尔公司All rights reserved.
Quantum dots emerge as an attractive alternative to small molecule fluorophores as fluorescent tags for in vivo cell labeling and imaging. This communication presents a method for specific labeling of live cells using quantum dots. The labeling is mediated by HaloTag protein expressed at the cell surface which forms a stable covalent adduct with its ligand (HaloTag ligand). The labeling can be performed in one single step with quantum dot conjugates that are functionalized with HaloTag ligand, or in two steps with biotinylated HaloTag ligand first and followed by streptavidin coated quantum dots. Live cell fluorescence imaging indicates that the labeling is specific and takes place at the cell surface. This HaloTag protein-mediated cell labeling method should facilitate the application of quantum dots for live cell imaging. (C) 2008 Elsevier Inc. All rights reserved.