Evaluation of co-delivery of colistin and ciprofloxacin in liposomes using an in vitro human lung epithelial cell model
Evaluation of co-delivery of colistin and ciprofloxacin in liposomes using an in vitro human lung epithelial cell model
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DOI：
10.1016/j.ijpharm.2019.118616
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发表时间：
2019-10-05
影响因子：
5.8
通讯作者：
Zhou, Qi (Tony)
中科院分区：
文献类型：
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作者：
Chai, Guihong;Park, Heejun;Zhou, Qi (Tony)
多重耐药革兰氏阴性菌引起的呼吸道感染给公众带来严重负担。我们之前的研究结果表明，与单一疗法相比，通过脂质体共同负载粘菌素和环丙沙星可改善针对多重耐药铜绿假单胞菌的体外抗菌活性。本研究旨在利用体外Calu-3细胞单层（空气界面条件下培养的肺上皮模型）研究粘菌素和环丙沙星在脂质体中的转运行为。细胞活力结果表明，在该浓度下单药或联合给药的细胞没有出现明显的毒性。
Respiratory tract infections caused by multidrug-resistant Gram-negative bacteria are serious burdens to the public. Our previous findings indicated that co-loading of colistin and ciprofloxacin via liposomes improved in vitro antimicrobial activities against multidrug resistant Pseudomonas aeruginosa as compared to the monotherapies. The current study aims to investigate the transport behavior of colistin and ciprofloxacin in liposomes using the in vitro Calu-3 cell monolayer, which is a lung epithelial model cultured under the air-interfaced condition. The cell viability results demonstrated that there was no obvious toxicity of cells exposed to single or co-administered drugs at the concentration