Induction of the Bacillus subtilis SOS-like response by Escherichia coli RecA protein.
Induction of the Bacillus subtilis SOS-like response by Escherichia coli RecA protein.
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大肠杆菌 RecA 蛋白诱导枯草芽孢杆菌 SOS 样反应。
DOI：
10.1073/pnas.83.14.5204
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发表时间：
1986
影响因子：
11.1
通讯作者：
Yasbin,RE
中科院分区：
文献类型：
--
作者：
Love,PE;Yasbin,RE
表达大肠杆菌RecA蛋白的质粒部分恢复了枯草芽孢杆菌recE4突变体中的DNA修复和重组能力以及诱导SOS样（SOS）反应。在DNA损伤剂存在下，E. coli RecA蛋白诱导din操纵子表达、Weigle再激活活性和a B的合成。枯草杆菌重组蛋白（Recbs）类似于RecA，但不能刺激原噬菌体诱导。此外，RecA蛋白能够在B的感受态recE4菌株中诱导E2应答。枯草芽孢杆菌，独立于暴露于DNA损伤剂。结果表明：（1）E.大肠杆菌和B.从表型和调节的观点来看，枯草芽孢杆菌中的RecA和莱克萨蛋白类似物惊人地相似，并且RecA和B中存在LexA蛋白类似物。枯草芽孢杆菌，（ii）Recbs蛋白能够调节其自身的产生，和（iii）在B中产生SOS诱导（RecA激活）信号。枯草杆菌的DNA损伤或生理能力的发展。
A plasmid that expresses the Escherichia coli RecA protein partially restored DNA repair and recombination capability and induction of the SOS-like (SOB) response in a recE4 mutant of Bacillus subtilis. In the presence of DNA-damaging agents, the E. coli RecA protein induced din operon expression, Weigle-reactivation activity, and synthesis of a B. subtilis recombination protein (Recbs) analogous to RecA but was unable to stimulate prophage induction. In addition, the RecA protein was capable of inducing the SOB response in competent recE4 strains of B. subtilis, independent of exposure to DNA-damaging agents. The results suggest that (i) the SOS response of E. coli and the SOB response of B. subtilis are strikingly similar from both a phenotypic and a regulatory standpoint and that RecA and LexA protein analogs exist in B. subtilis, (ii) the Recbs protein is capable of regulating its own production, and (iii) SOS-inducing (RecA-activating) signals are generated in B. subtilis following either DNA damage or the development of physiological competence.